linking MS/MS spectra, genomic associations, and clinical traits that can be leveraged for annotation and identification of unknown metabolites implicated in diverse disease processes. Our integrative omics approach highlights the value of whole genome sequencing analysis of the metabolome in diverse ...
S11). Subsequently, the PCC analysis (Fig. 3D) and PLS-DA (Fig. 3E) were performed based on the calculated content (Additional file 1: Table S8). Clearly, the PCC results can not differentiate the normal and abnormal samples at the threshold value of 0.9, while in total twelve samples ...
with linear discriminant analysis (LDA) score of > 2.5 andPvalue of < 0.05. BT, 6 cattle of control group at 1 day before transport; ACon
The correlation analysis was conducted using Pearson’s correlation coefficient in the R package to calculate the correlation coefficient Mantel’srandPvalue (Mantel’sP) of genes and metabolites in the PF pathway. To identify the regulatory relationships of gene expression in the PF pathway, transcrip...
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However, there are some false-positive results while performing an IgM-IgG immunoassay because (1) some weak positive results near the Cut-off value are likely to be misinterpreted as false positive; (2) the presence of endogenous or exogenous interfering substances in patient samples could lead ...
product analysis by mass spectrometry. Combining these features with acoustic droplet ejection mass spectrometry we could eliminate slow chromatographic steps and continuously evaluate chemical reactions in multiplexed formats. This enabled the assignment of reaction conditions to molecules derived from ultrahigh...
Analysis of samples was performed using anion-chromatography coupled directly to high-resolution orbitrap mass spectrometry69. A 5 μl partial loop injection was used for all analyses and chromatographic separation was performed using a Dionex ICS-5000+ Capillary HPIC system (Thermo Scientific, San...
Mutant KRAS (KM), the most common oncogene in lung cancer (LC), regulates fatty acid (FA) metabolism. However, the role of FA in LC tumorigenesis is still not sufficiently characterized. Here, we show that KMLC has a specific lipid profile, with high tri
Tumor 1 was excluded from this analysis because of its outlier nature (see Fig. 1B and Discussion). We used average fold changes to calculate a two components model (as performed for individual HCC analysis), and extracted proteins with q value < 0.2 (considered as differentially expressed, ...