Microbial consortia composed of autotrophic and heterotrophic species abound in nature, yet examples of synthetic communities with mixed metabolism are limited in the laboratory. We previously engineered a model cyanobacterium, Synechococcus elongatus PC
N protein is among the most abundant SARS-CoV-2 proteins9. As ORFNis immediately upstream ofORF10inNsgRNA, translation ofNcould disrupt RNA structures driving internal initiation ofORF10, thereby facilitating re-initiation ofORF10in the 3′-UTR, as anNsgRNA-specific route forORF10translation41. ...
Additional file1: Figure S2). In all consortia,cscB+S. elongatusis inoculated with a heterotrophic microbe in the appropriate co-culture media (with or without the addition of 1 mM IPTG to inducecscBexpression; see Materials and Methods) and grown over 48 hours in...