This review summarizes common algorithms and tools in RNA computational biology, with a focus on applications of expert system, machine learning, and deep learning to ncRNA identification, ncRNA target prediction, RNA modification, RNA secondary structure detection, RNA-protein interaction and RNA ...
Firstly, we profiled the landscape of the G-rich regions with the potential to fold into RG4s in vitro in the Arabidopsis transcriptome. We also revealed the unique RNA structural features of these regions. Using chemical structure probing, we found that those G-rich regions with in vitro ...
DMS-MaPseq enables genome-wide and target-specific RNA secondary structure probing of even rare or heterogeneously structured RNAs in vivo and was used to study structure involved in translation regulation as well as nascent transcripts. Coupling of stru
Global Mapping of Human RNA-RNA Interactions Molecular Cell, Volume 62, Issue 4, 19 May 2016, Pages 618-626 Eesha Sharma, Tim Sterne-Weiler, Dave O’Hanlon, Benjamin J. BlencoweView PDF Referred to by Direct Duplex Detection: An Emerging Tool in the RNA Structure Analysis Toolbox Trends...
RNA structure probing uncovers RNA structure-dependent biological functions Article 25 June 2021 Change history 09 August 2019 The legend of Figure 6 was amended to specify that ref.144, from which part a is adapted, is published with open access under the CC-BY-4.0 license (https://creati...
performance. Inspecting the components of such batteries is useful when verifying material quality in the assembled cell and seeing the impacts of power cycling on material structure. Secondary cathode particles in lithium-ion batteries can be seen in 3D with FIB-SEM. Cracks ...
Conversely, DMS readily penetrates cell walls and membranes, making DMS a crucial tool for in vivo RNA structure probing, but it is limited in providing information only for the Watson–Crick face of A and C, and the Hoogsteen face (N7) of G. Our review discusses recent advances of DMS ...
Downstream bioprocessing is a series of processing steps to isolate and purify the molecular target from the cell. Raman spectroscopy as a process analytical technology (PAT) in bioprocessing Advances in cell engineering, process control, and media composition are credited with improving the volumetric ...
b, DMS-MaPseq probing of the intracellular HIV-1NL4-3 RRE in CD4+ T cells was used as input for DREEM clustering. Two clusters passed the BIC test and were used as constraints on the folding using RNAstructure. Structural models are colour-coded by normalized DMS reactivity; bases not ...
Cell Research volume 32, pages 157–175 (2022)Cite this article 24k Accesses 11 Altmetric Metrics details Abstract A major obstacle in Alzheimer’s disease (AD) research is the lack of predictive and translatable animal models that reflect disease progression and drug efficacy. Transgenic mice ...