In accordance with the present invention, an improved anion- exchange chromatographic composition has been formed which comprises synthetic resin support particles and anion exchange compounds attached thereto, wherein the anion-exchange compounds comprise an internal reactive group, which may be an ester...
Fractionation of nuclear extracts from a multi-drug-resistant subclone of the human promyelocytic subline HL-60 by anion-exchange chromatography and chromatofocusing resolves at least two different subtypes of topoisomerase II, which are not identical to the known α- and β-forms of the enzyme beca...
It is apparent that living bacteria cells exhibited similar behaviors and properties with molecules in anion exchange chromatography, implying that chromatographic methods can become an effective approach in the studies of intact bacterial cells. 展开 ...
Crude enzyme solutions were initially submitted to anion exchange chromatography on a HiTrap Q HP column (1.6 cm × 2.5 cm, 8.6 µm, 5 mL, GE Healthcare). In the first round, 50 mM Tris–HCl buffer at pH 7.4 was used as the equilibration buffer. Targeted enzymes were...
They are composed of three highly polar subunit molecules (a nucleobase, a sugar, and at least one phosphate group), which makes their separation and analysis very challenging by conventional liquid chromatography techniques. Herein, we describe a simple, efficient, and cost-effective ion-exchange ...
Metabolomics using anion-exchange chromatography–mass spectrometry To measure metabolite abundances under conditions at which insulin secretion takes place, 50 islets from control and diabetic mice were preincubated for 1 h in 2 mM glucose Krebs–Ringer bicarbonate buffer + 0.1% (w/v) FFA...
This antioxidant activity was purified by DEAE anion exchange chromatography to a fraction (called IE-1) that was comprised of a single main protein band... A Boonmee,C Srisomsap,A Karnchanatat,... - 《Food Chemistry》 被引量: 48发表: 2011年 Molecularly imprinted solid-phase extraction of...
The separation of fluoride ions from interfering anions and cations by anion exchange chromatography Fluoride may be separated from phosphate and the anionic metal chelates of ethylenediamine-tetraaceticacid(EDTA) by absorption on, and elution from, an ani... ACD Newman - 《Analytica Chimica Acta》 ...
ChemInform Abstract: Anion Exchange Reactions in Layer Structured Crystalsinorganic chemistry, reviewinterfaces, interface equilibria, chromatographyChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform ...
analyte classes, using a mixed mode cartridge (reverse phase, π–π bonding, strong anion exchange), then α-tocopherol and cholesterol were measured using standard techniques, while the fatty acids were quantitated using a novel, reverse phase liquid chromatography–mass spectrometry (LC–MS) ...