Preparative anion-exchange chromatography of soybean trypsin inhibitor: the alternative of column-overload methodsdoi:10.1016/0378-4347(92)80016-JThe objective of preparative separation is to purify the largest amount of material in the shortest time and at a minimum cost, i.e. to maximize ...
Format/Principle:Anion-exchange chromatography (Gravity-flow column) Lysate Clarification:Filtration Sample Size:120~240 ml of bacterial culture for high/low-copy number plasmids. Plasmid or Constructs Range:3 kbp~150 kbp Binding Capacity:≤1.5 mg DNA/Column ...
2.1.2 Anion exchange chromatography Anion exchange chromatography (AEC) is also a classical approach to purifying free polySia and related bioconjugates, such as polysialylated-proteins, peptides and glycans from neutral and alkaline components in complex matrices. It utilises the principle of electrost...
20. Protein a can be applied to the anion exchange chromatography matrix by methods well known in the art. 通过本领域公知的方法可以将蛋白a施加到阴离子交换色谱基质上。 youdao 21. The lean solvent sulfolane processing amount were larger than 300per mass of the anion-exchange resin. 用阴离子交换...
Omp32 was solubilized with octyl-polyoxyethylene (OPOE) and purified by gel-filtration and anion-exchange chromatography as described previously [26]. The porin was dialyzed against crystallization buffer, containing 2% β-d-octylglucoside to exchange the detergent, and crystallized as described in ...
Silica‐based monolithic columns with mixed‐mode reversed‐phase/weak anion‐exchange selectivity principle for high‐perfor... Lindner.Silica-based monolithic columns with mixed-mode reversed-phase/weak anion-exchange selectivity principle for high-performance liquid chromatography.J Sep... R Nogueira,D...
Anionexchange resins (AER) have been used to determine plant available phosphorus (P) since the fifties and their results have shown strong relationships with plant growth and P uptake irrespective of soil properties. However, this procedure is still not widely used by laboratories because of diffic...
Bipolar membranes (BPM) consist of an anion exchange layer, a cation exchange layer, and a thin hydrophilic polymeric interlayer between the two, which is usually referred to as the interfacial layer. From: Desalination, 2023 About this pageAdd to MendeleySet alert ...
Lake JE, Renner EL, Scharschmidt BF, Cragoe EJ, Hagey LR, Lambert KJ, Gurantz D, Hofmann AF (1988) Inhibition of Na+/H+ exchange in the rat is associated with decreased ursodeoxycholate hypercholeresis, decreased secretion of unconjugated ursodeoxycholate, and increased ursocholate glucuronidati...
(using Human New York anti sera isolated from HIV infected patients) prepared by the perfusion bioreactor system and isolated using inactivation, filtration and anion exchange chromatography steps (lanes 2-5 respectively) and the staining profile for the reference standard lot of HIV-1 particles at ...