Disclosed are compositions and methods for measuring the likelihood of recovery of a recently thawed immune cell. Methods include assaying the level of an ADAM-17-cleaved surface receptor expressed on the immune cells, wherein the level of the surface receptor directly correlates with the likelihood...
Targeting immune checkpoint protein interactions is one form of cancer immunotherapy that offers a novel way to attack tumor cells. The discovery of those druggable protein targets relies on an in-depth understanding of cell signaling pathways...
GO analysis showed that genes were principally enriched in the following biological processes: immune cell activation, humoral immune response, and immune response regulation (Fig. 1D). Figure 1 Identification of genes related to immunotherapy treatment. (A) As a result, two non-grey modules are ...
We next sought to delineate mechanisms behind the site-specific reduction of IgA+cells in the colon and colonic-associated MLN during HFD feeding. Using a custom gene expression assay containing secreted factors that mediate plasma cell differentiation and/or IgA class-switch recombination (CSR), we...
8E). The wound-healing assay showed that ZC3H13 knockdown significantly enhanced the invasion ability of HeLa and SiHa cells (Fig. 8F). Transwell experiments showed that ZC3H13 knockdown significantly enhanced the cell migration ability (Fig. 8G). The wound-healing assay showed a significant ...
Targeting Treg cells with GITR activation alleviates resistance to immunotherapy in murine glioblastomas. Nat Commun. 2021;12(1):2582. CAS PubMed PubMed Central Google Scholar Dubinski D, Wölfer J, Hasselblatt M, Schneider-Hohendorf T, Bogdahn U, Stummer W, Wiendl H, Grauer OM. CD4 +...
In PCR assay, Lnc00857 was successfully knocked down in A549 and Calu3 cells after siLnc00857 transfection (Additional file 4C). The CCK-8 and EdU assay were conducted to provide cell viability (Fig. 2B) and proliferation (Fig. 2C) evaluation. In the siLnc00857 group, decreased cell ...
In contrast, apoptotic cell death is “clean” in general and not immuno-stimulatory. Many unnecessary cells to be removed during development are cleared out through apoptosis (Miura, 2011). Apoptotic stimuli lead to the activation of caspases, predominantly in a protein complex called apoptosome....
NK cell cytotoxicity and activation assay GBM43, BT422, U251T2, GBM30, LN229, and Gli36ΔEGFR cells were used as target cells. Primary human NK cells isolated from leukocyte cones of healthy donors using an NK cell isolation kit (MACSxpress Miltenyi Biotec, San Diego, CA) and an erythroc...
The proliferation, viability and expression of activation markers were assessed by flow cytometry from Day 0 (before adding 19K-6H) to Day 4. The 19K-6H polymer probe did not induce either short-term or long-term cell mortality. More than 70% of the cells remained alive 15 h post-...