An immortalized human cell line is provided which has the characteristics of human embryonic microglia. Such immortalized microglia cells express CD68, CD11c and MHC class I and II antigens as surface markers;
永生化人小胶质细胞(SV40转染细胞系)来源于原代人小胶质细胞(纯度99%),并保留了小胶质细胞特异性标记,如Iba1、CD68、TREM2和CD11b。这些细胞适用于研究人类小胶质细胞在健康和疾病中的作用。 Immortalized Human Microglia (SV40 cell line) is derived from Primary Human Microglia Cells (>99% purity) and have...
Cell Morphology :Polygonal Seeding Density:25,000 - 30,000 cells per cm2 Population Doubling Time:28 - 38 hours Reporter: GFP Unit quantity :1x10^6 cells / 1.0 ml Propagation Requirements:The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To ...
In addition to the infiltration of peripheral immune cells, neonatal HI induces a strong local inflammatory response, characterized by an increased microglia accumulation and activation [59]. Since dense accumulation of microglia in severely affected HI-injured animals challenges single-cell counting (Supp...
To study microglia's physiological and pathological functions, one of the most widely used models is primary microglia culture from neonatal rodents. However, primary microglia culture is time consuming and needs a great number of animals. In our microglia culture, we found a strain of ...
However, immortalization by genetic or pharmacologic manipulations may show altered physiology from primary microglia. A novel microglial cell line was isolated from a primary glial culture of postnatal murine cerebral cortices. The culture contained a population of spontaneously transformed microglia that ...
Therefore, a pathophysiologically relevant cell line derived from a natural TSE host could be used for propagation of natural prion isolates. In this study, ovine brain macrophages (microglia) were immortalized by transfection with the human telomerase reverse transcriptase (hTERT) gene to identify ...
In the present work, we carried out an extensive characterization of Immortalized Human Microglia鈥擲V40 cell line (IMhu), marketed by Applied Biological Material. The functional response of IMhu to a large variety of stimuli was studied. In particular, we investigated morphology, mortality, and ...
Therefore, a pathophysiologically relevant cell line derived from a natural TSE host could be used for propagation of natural prion isolates. In this study, ovine brain macrophages (microglia) were immortalized by transfection with the human telomerase reverse transcriptase (hTERT) gene to identify ...