A 1 ml syringe with a 34-gauge needle was used to penetrate the tympanic membrane to inject 100 mg ml−1 sodium arsanilate (A9528 Sigma-Aldrich, 40 μl, prepared in 0.3 M NaHCO3) into the middle ear. Mice were left undisturbed for one week before behavioral tests. The...
After that, the mixture was drawn into the microfluidic channel by a syringe pump, and the flow rate was kept at 5 μL min−1. It should be noted that when the flow rate is too low, the AuNPs might not flow consistently through the microfluidic channel, as indicated by the arrow in...
Up to 5 μl of PBNC-labeled MSCs suspended in PBS at 10k cells/μl were directly injected into the spinal cord per the clinical procedure at a rate of 16 nl/sec using a 33 G syringe attached to an ultra-micropump (World Precision Instruments). 2.6. Real-time US/PA imaging during ...
Fluid movement within a microfluidic device can be driven passively through capillary forces within the microchannels or actively using externally applied forces such as pressure from syringe pumps [263]. Additional elements such as valves and flow resistors can be incorporated to regulate the flow ...
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