Protein biomarkers have been broadly investigated in cerebrospinal fluid and blood for the detection of neurodegenerative diseases, yet a clinically useful diagnostic test to detect early, pre-symptomatic Alzheimer’s disease (AD) remains elusive. We con
Cultures (3 mL) were centrifuged at 1500×g for 5 min, the supernatant was filtered through GD/X 25 mm syringe filter (polyvinylidene difluoride filtration medium, 0.2 µm, GE Healthcare Life Sciences, Cytiva, Marlborough, MA) and 1 mL of cell free supernatant was aliquoted into sterile ...
Schematic diagram of the MSFI-BI-LOV manifold hyphenated with LC for the determination of trace level concentrations of acidic drugs. LOV, lab-on-valve, MP, multisyringe; Si, syringe; SV, solenoid valve; HC, holding coil; CC, communication channel; C1 and C2, cavities for beads; P, ...
P2 virus was generated using one volume of P1 virus added into 100 volumes of fresh culture at a density of 1.0 × 106 cells/mL. P2 virus was harvested 3 days afterwards, as for P1 virus, except the supernatant was filtered through a 0.45 µm PVDF syringe filter. P3 virus,...
After reaction for 2 min, approxi- mately 8 mL sample was taken out and passed through a 0.45 μm membrane filter syringe to immediately separate catalysts from the solution. The diphenycarbazide (DPC) colorimetric method22 with JASCD V-670 spectropho- tometer and the ion ...
An aliquot equal to 0.3 cc was measured in a 1.0-cc syringe and placed into each posterolateral gutter spanning the transverse processes for a total of 0.6 cc of graft per animal. The wound was irrigated and closed. High-resolution radiographs were obtained at 1 month. The presence or ...
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All compounds and 5-FU have been dissolved in DMSO to prepare stock concentrations of 5 mg/ml. The stock solutions were filtered via 0.22 lm syringe filter. Double fold dilutions were prepared through including equal volumes of the dissolved compounds to fresh RPMI-1640 culture medium. Ninety-...
Microfat: 15 cc of fat obtained was processed by an intersyringe shuffle using a Luer to Luer 2.4 mm Anaerobic Transfer (Tulip Medical, San Diego, CA, USA). After 20 passes, the same process was repeated through Luer-Lock 1.2 mm Connector (Tulip Medical, San Diego, CA, USA) for the...