To this aim, both WT and mutant PSEN1 ChLN cultures were exposed to ACh (1 mM final concentra- tion). Fig. 8 shows that ACh induced an elevation of intracellular C a2+ in WT PSEN1 ChLNs at day 0 (Fig. 8A,E, average fluorescence change (ΔF/F) = 2.58 ± 0.22), and ...