目前肿瘤免疫疗法CAR-T发展如火如荼,但是依然存在阻碍,如T细胞寿命短,效力不够等,这些问题严重阻碍了CAR-T治愈肿瘤的功效,特别是在实体瘤根治上。鉴于细胞因子的抗肿瘤功能,不难想到,也许共表达这些细胞因子会解决这一难题,并有望成为攻克实体肿瘤的重要手段。 如第四代CAR-T:在第二/第三代CAR-T 的基础上共表...
【3】 Zhou J, Jin L, Wang F, Zhang Y, Liu B, Zhao T. Chimeric antigen receptor T (CAR-T) cells expanded with IL-7/IL-15 mediate superior antitumor effects. Protein Cell. 2019 Oct;10(10):764-769. 关于同立海源 北京同立海源生物科技有限公司,成立十余年专注细胞和基因治疗(CGT)上游GMP...
如第四代CAR-T:在第二/第三代CAR-T 的基础上共表达一些关键的细胞因子中表现出了克服实体瘤免疫抑制微环境的能力。 以IL-12为例,系统注射IL-12会引起严重的炎症副反应,但是通过共表达IL-12的第四代CAR-T细胞来实现局部表达后,可以在肿瘤病灶处招募NK细胞,或者直接逆转被耗竭的肿瘤浸润性T淋巴细胞,从而有效克...
Infiltration, accumulation, and survival of chimeric antigen receptor T (CAR-T) cells in solid tumors is crucial for tumor clearance. We engineered CAR-T cells to express interleukin (IL)-7 and CCL19 (7 × 19 CAR-T cells), as these factors are essential for the maintenance of T-cell zo...
T-CELLSANTITUMOR IMMUNITYEXPRESSIONBackground Chimeric antigen receptor (CAR)-T-cell therapy is a revolutionary treatment that has become a mainstay of advanced cancer treatment. Conventional glypican-3 (GPC3)-CAR-T cells have not produced ideal clinical outcomes in advanced hepatocell...
如第四代CAR-T:在第二/第三代CAR-T的基础上共表达一些关键的细胞因子中就表现出了克服实体瘤免疫抑制微环境的能力。 以IL-12为例,系统注射IL-12会引起严重的炎症副反应,但是通过共表达IL-12的第四代CAR-T细胞来实现局部表达后,可以在肿瘤病灶处招募NK细胞,或者直接逆转被耗竭的肿瘤浸润性T淋巴细胞,从而有效克...
Combination of 4-1BB and DAP10 promotes proliferation and persistence of NKG2D(bbz) CAR-T cells Authors - Cheng Wei View Article Biochem J Proteomic analysis in primary T cells reveals IL-7 alters T cell receptor thresholding via CYTIP/cytohesin/LFA-1 localisation and activation Authors - Rayne...
Briefly, PBMC were activated by immobilized CD3/C28 mAbs and transduced with retroviral supernatant encoding the CAR.CD19.CD28ζ. During the activation, transduction and expansion, T cells from 7 donors were exposed to either IL2 (50 U/mL), IL7 (10 ng/mL), IL15 (5 ng/mL) or the ...
CAR-T疗法的另一个挑战是由IL-6大量分泌引起的细胞因子释放综合征(CRS),目前临床上使用Tocilizumab来减少CRS的发生,但仍存在很多副作用。 基于此,该团队构建了一种CD3抗体修饰的脂质纳米粒(AntiCD3-LNP/CAR19+shIL6),包裹含有白细胞介素6短发夹RNA ...
lefrère,f.,braudeau,c.,brouard,s.,soulillou,j.-p.,和laplaud,d.-a.(2008).当从分析中排除表达il-7受体α链的细胞时,具有复发缓解型多发性硬化的患者具有正常的treg功能(patientswithrelapsing-remittingmultiplesclerosishavenormaltregfunctionwhencellsexpressingil-7receptoralpha-chainareexcludedfromthe...