Knockdown efficiency of IL‐17RA siRNA was confirmed by a reduction (55%) of IL‐17RA mRNA level in the PVN. Additionally, the mRNA levels of TNF‐α (1.12 ± 0.24 vs. 0.51 ± 0.11*), IL‐1β (1.05 ± 0.16 vs. 0.64 ± 0.09*), and MCP‐1 (1.01 ± 0.10 vs. 0.60 ± 0.10*...
5e–g). Meanwhile, the expression of collagen and α-SMA were also reduced in HSFs transfected with IL-17RA siRNA, which was in accordance with the effect of miR-192-5p mimics on HSFs, and there were significantly statistical differences compared to their negative controls (p < 0.05) ...
CST 中国北京特约代理 eBioscience 中国北京特约代理 Roche 中国北京特约代理 实时荧光定量PCR(Real-time PCR)技术服务 RNA干扰服务 siRNA合成服务 RNAi载体构建服务 质粒载体RNA干扰服务 慢病毒RNA干扰服务 腺病毒RNA干扰服务 miRNA服务 miRNA定量服务 miRNA功能研究服务 miRNA测序服务 蛋白表达纯化服务 哺乳动...
To better understand IL-17RA signaling, we undertook a high-throughput siRNA screen in IL-17A stimulated human lung epithelial cells to identify novel signaling molecules in this pathway. In addition, since many of the signaling proteins reported to be used by IL-17RA are also used in the ...
IL-17RA预后目的探讨IL-17RA在人肝细胞癌(hepatocellular carcinoma,HCC)组织中表达情况及其对肝肿瘤切除患者预后的影响,并评价其对HCC细胞株生物学特性的影响.方法收集163例HCC组织并制成组织芯片,免疫组化染色法检测肿瘤组织中IL-17RA的表达情况,Kaplan-Meier法和Cox回归模型分析患者总生存期的影响因素.采用siRNA...
survival for HCC patients underwent resection.The abilities of migration and invasive of MHCC-97H and Huh7 were decreased after down-regulated the expression of IL-17RA,and the inhibition rate of oxaliplatin on tumor cells was increased after down-regu...