MHCIIhi DCs and MHCIIint DCs correspond to respectively migratory DCs and lymph node resident DCs and were identified based on their relative expression of CD11c and MHCII after exclusion of the Ly6Chi monocyte population (Fig. 1F). The absolute cell numbers of Ly6Chi monocytes, migratory DCs...
作者根据标记物 MHCIIhi CD11chi CD11b int-high(在粘附和非粘附细胞中)的表达对细胞进行分拣,得到三个群体: 然后作者将其与 BMDMs 的 Il12b-YFP 表达水平进行了比较。然后,作者比较了 BMDM-Il12b-YFP 亚群、GM-DCs 和 GM-Macs 以及 BMDM 未处理的 M0 对照之间的巨噬细胞和 DC 相关表面标记物。经 LPS 或...
(C) Flow cytometric analysis of CD11c+F4/80−MHCII+CD103−CD11b+ DCs (left) or CD11c+F4/80−MHCII+ CD11b−CD103+ DCs (right) in tumors from the MMTV-PyMT animals shown in Figure 2A. Significance was determined by an unpaired t test relative to IgG1 or IgG1/PTX control grou...
Furthermore, more than 70% of IL-12-producing cells could be identified as CD45+Lin−CD88−MHCII+CD11c+CD11b−XCR1+ cells, consistent with the canonical description of cDC1s28 (Figure S3A). We did not observe significant CD45+Lin−CD88+YFP+ macrophage or CD45+Lin−CD88−...