Total RNA was isolated from highly pure, sorted human ILC subsets using the TRIZOL reagent according to the manufacturer’s instructions (Invitrogen). Final preparation of RNA was considered DNA- and protein-free if the ratio of readings at 260/280 nm was ≥1.7. Isolated mRNA was reverse-tr...
(Sense: 5′-UUCUCCGAACGUGUCACGUTT-3′; Antisense: 5′-ACGUGACACGUUCGGAGAATT-3′) (Genepharm, Shanghai) for 72 h using lipofectamine RNAiMAX transfection reagent (Thermo Fisher) according to the manufacturer’s instructions (https://www.thermofisher.cn/order/catalog/product/13778075?SID=srch...
ELISA detection of human IFN-γ was conducted following the instructions (BioLegend). T cells proliferation assay TCR-T were labeled with Carboxyfluorescein succinimidyl amino ester (CFSE) following the instruction of CellTrace™ CFSE Cell Proliferation Kit (Thermo Fisher Scientific, Waltham, MA, ...
Fc–IL-4 enhances type 1 immunotherapies We next investigated whether Fc–IL-4 as a type 2 cytokine could enhance the antitumour efficacy of type 1-centric ACT and ICB therapies against solid tumours (Fig.2). In the B16F10 mouse melanoma model (Fig.2a), ACT of PMEL T cells (5 ×...
Immunoreactive bands were enhanced with ECL for chemiluminescence (see manufacturer's instructions). The experiment was repeated thrice. The average gray value of the scanned bands was determined by image-J. 3.6. Loading with mechanical compressive stress In this experiment, a self-developed cell ...
To get a list of programs running, please follow these instructions:- Start->run- type "cmd" and click "OK"- on the black window that opens, type "tasklist /svc" and press enter- right click on the window title and select Edit->Mark- Scroll up to the begining of the...
Immune checkpoint inhibitors (CPIs) are a relatively newly licenced cancer treatment, which make a once previously untreatable disease now amenable to a potential cure. Combination regimens of anti-CTLA4 and anti-PD-1 show enhanced efficacy but are prone to off-target immune-mediated tissue injury...
according to the manufacturer’s instructions. Reverse tran- scription and real-time PCR reactions were then carried out using LightCycler-RNA Master Hybridization Probes (one- step procedure) on a LightcyclerR apparatus (Roche Diagnostics, Brussels, Belgium). Primers, standards, and real-time PCR...
Briefly, ~80 ng of total RNA was isolated and hybridized with reporter and capture probes in custom-made T helper cell-targeted nCounter Gene Expression code set according to manufacturer’s instructions (NanoString Technologies). Data were analyzed using nSolver Analysis software63. In vitro ...
cDNA was synthesized using High Capacity cDNA Reverse Transcription kit (Life technologies) according to the manufacturer's instructions. Real-time quantitative PCR was performed using validated TaqMan Gene Expression primer and probe sets (Life Technologies) according to the manufacturer's instructions. ...