we speculated whether other WAT-resident cells could be the major targets of IL-1β secreted from adipose tissue macrophages26. To investigate how the absence of IL-1 signaling affects WAT expansion, we examined whole-body IL1R1-knockout (KO) mice and WT controls. Despite ...
For binary outcomes, C is identical to the area under the receiver operating characteristic (ROC) curve, or a plot of sensitivity (SN) versus one minus specificity (1-SP) of the test in question. Antigen-presenting cell Cell which processes and presents antigen on its cell surface to ...
b Spectral flow analysis of Ba-infected omenta showing IL-1RA expression profiles of the myeloid cells indicated at Day 8 p.i., together with their fluorescence minus one (FMO) control (top). Normalized absolute numbers of these omental IL-1RA+ Ba-infected (red) or PBS-treated (black) ...
we engineer a recombinant human IL-33 protein that expands intratumoural lymphoneogenic ILC2s and TLSs and demonstrates enhanced anti-tumour activity in PDAC mice. In summary, we identify the molecules and cells of a druggable pathway that induces inflammation-triggered...
The area of tissue loss was calculated as the area of the contralateral hemisphere minus the non-infarcted area of the ipsilateral hemisphere. Brain infarct volume was determined by multiplying the area of tissue loss by the distances between the analyzed brain sections. Bone marrow isolation and ...
A-tailing was then performed by adding 5 μL of 10x Klenow Buffer (NEB), 1 μL of dATP (10 mM) and 1 μL of exo minus Klenow (5U/μl, Fermentas); the reaction was incubated at 37°C for 30 min and purified using MinElute PCR purification columns (QIAGEN) and cDNA eluted in ...
3A and B). The staining of M1 and M2 was confirmed with the fluorescence minus one (FMO) controls (Fig. S6). Collectively, these results demonstrated that pristane contributes to the polarization of macrophages toward the M2 phenotype systemically during atherosclerotic lesion formation. 3.4. ...
Cell Communication and Signaling. 2020;18:137. Article CAS PubMed Google Scholar Wang A, Si Z, Xue P, Li X, Liu J. Tacrolimus protects hippocampal neurons of rats with status epilepticus through suppressing oxidative stress and inhibiting mitochondrial pathway of apoptosis. Brain Res. 2019;...
Nuclear translocation was measured using the “Cytoplasm to Nucleus Translocation Application” software (Image analysis system, ArrayScan HCS System, Cellomics™) Nuclear translocation units represent the measurement of the average intensity of the target in the nuclear region, minus the average intensity...
Objective To compare the effect of Haizao Yuhu Decoction and its recipe minus Sargassum and/or Glycyrrhiza on mT0R-p70S6K/4E-BP1 signaling pathway in rats with goiter. Methods Eighty-four male Wistar rats were randomly divided into blank group, model ...