免疫组化是应用免疫学抗原与抗体特异性结合的原理,通过化学反应使标记抗体的显色剂(荧光素、酶、金属离子、同位素)显色,从而确定组织细胞内抗原(多肽和蛋白质),对其进行定位、定性及相对定量的研究,称为免疫组织化学技术(immunohistochemistry),锐博生物可提供IHC免疫组化染色服务。
HE染色与IHC染色的区别,你了解多少? 苏木精—伊红染色法(hematoxylin-eosin staining),简称HE染色法,是石蜡切片技术中常用的染色方法之一。苏木精染液呈碱性,主要使细胞核内的染色质和胞质内的核酸染成紫蓝色;伊红染液呈酸性,主要使细胞质和细胞外基质中的成分染成红色。HE染色法是组织学、胚胎学、病理学教学与科研中...
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Discover why Immunohistochemistry (IHC) is an important application of histology. Learn steps & best practices for better IHC staining with our in-depth guide.
Vener Materials Catalog# Haematoxylin Eosin (H&E) staining PH0516 二甲苯 无水乙醇 01M柠檬酸钠缓冲液 30%过氧化氢 实验流程与方法：脱蜡与水化：将石蜡切片置于60℃烤箱中烤片30-60分钟，以促进蜡片水分的蒸发和石蜡的融化，从而确保组织切片能牢固地附着在玻片上。接着，依次将切片放入二甲苯Ⅰ、Ⅱ、Ⅲ中...
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Basic IHC staining typically requires sample preparation, antigen retrieval, blocking, target detection, and visualization. Here we summarize the basic steps for IHC and the tools available for obtaining publication-quality images. Find IHC antibodies On this page: 5 Steps for great IHC images IHC ...
Traditional Chromogenic IHC Staining and Detection. As illustrated above, enzymatic conversion of a chromogen substrate is required to generate a signal and visualize an antigen in traditional IHC assays. Fluorescence detection is an alternative method that is more suitable for multicolor experiments due ...
[2] Amereh M,et al.Immunohistochemistry (IHC) staining of in-vitro cancer cell-generated tumoroids. MethodsX. 2023 Jun 3;10:102242. [3] Shi SR,et al.Antigen retrieval in formalin-fixed, paraffin-embedded tissues: an enhancement method for immunohistochemical staining based on microwave oven he...