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Double or Nuclear Staining Detection Deparaffinization and Rehydration Tip: Before moving to alcohol grades step, make sure to completely deparaffinize the sections. If the sections still have traces of wax, an additional immersion of 5 minutes in Xylene may be employed. Deparaffinize and rehydrate...
Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections ...
Learn more about Immunohistochemistry (IHC) protocol for preparation and immunofluorescence staining, including tissue fixation and freezing. (142)
Recommended Protocol(s)1. When used with manual IHC staining techniques agitate slides 5 times in the wash buffer to remove excess staining reagents.2. When used with automated IHC staining techniques use according to the instrument manufacturer’s specifications.The clinical interpretation of any ...
Find Your IHC Staining Protocol: Chromogenic Staining of FFPE Tissue Protocol Chromogenic Staining of Frozen Tissue Protocol Fluorescent Staining of FFPE Tissue Protocol Fluorescent Staining of Frozen Tissue Protocol Secondary Antibodies and Detection Reagents ...
注意:小心不要引入任何气泡或扰乱样品。 5.使用显微镜对载玻片进行成像。 注意:如果不立即使用,请将载玻片存放在4°C的黑暗环境中。 阿拉丁化学试剂:https://www.aladdin-e.com/zh_cn/faq/protocols/immunohistochemical-staining-protocol-for-frozen-samples-ihc-fr/...
· 接下来的步骤按照常规使用的protocol进行 阴性对照:通过去除实验中的一抗,与标记的二抗孵育,可确认阻断剂的阻断效率。确认内源性信号确实被阻断剂抑制后,继续进行常规实验。 当样本的内源性IgG处于高水平时,可能需要将Fab抗体的浓度增加到100μg/ml。为了避免Fab抗体被标记的二抗取代,如果不影响目标蛋白的抗原性...
注意:小心不要引入任何气泡或扰乱样品。 5.使用显微镜对载玻片进行成像。 注意:如果不立即使用,请将载玻片存放在4°C的黑暗环境中。 阿拉丁化学试剂:https://www.aladdin-e.com/zh_cn/faq/protocols/immunohistochemical-staining-protocol-for-frozen-samples-ihc-fr/...
New, next-generation detection products for immunohistochemistry (IHC) feature improved DAB substrate reagents for greater signal sensitivity and the addition of an endogenous peroxidase quenching solution for a more complete IHC staining protocol. Incre