[0478] 25.Leung SCY,Nielsen TO,Zabaglo L,et al.Analytical validation of a standardized scoring protocol for Ki67:phase 3 of an international multicenter collaboration.NPJ Breast Cancer.2016;2:16014. [0479] 26.Harris LN,Ismaila N,McShane LM,Hayes DF.Use of biomarkers to guide decisions on ...
IHC Double Staining Guide:免疫组织化学双重染色指南 热度: 《免疫组织化学及相关技术》第二章2免疫组织化学技术 热度: 组织化学及免疫组织化学 热度: 1 Immunohistochemistry (IHC-P) Protocol Immunohistochemistry (IHC) is a method for demonstrating the presence and location of proteins in tissue sections. ...
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免疫化学( Immunochemistry),包含免疫组织化学( Immunohistochemistry, IHC)和免疫细胞化学( Immunocytochemistry, ICC),是利用抗原与抗体之间的结合具有高度特异性的原理,通过抗原抗体结合及呈色反应,显示组织或细胞中的化学成分,对组织切片或细胞标本中某些化学成分进行定性、定位或者定量研究。
Jump to: Immunohistochemistry Overview IHC preparation IHC On Paraffin Sections IHC on Frozen Tissue IHC Antigen Retrieval IHC Blocking Selecting Antibodies For IHC IHC Sample Visualization IHC Troubleshooting IHC ppt Immunohistochemistry Video Protocol
Download PDF version Immunohistochemistry On Paraffin Sections Please note: All steps in the following protocol are carried out at room temperature unless stated otherwise. Deparaffinizing and rehydration Immerse slides in xylene for 10 minutes. Repeat this step again in fresh xylene for a further 10...
Confirms if the protocol is correct. Validates negative results. Negative Control Control tissue that is not known to express the protein of interest. Ensures that the observed staining pattern is due to specific signals. Usewww.uniprot.orgorwww.pax-db.orgto find endogenous negative controls in ...
ClearConfirm Search Step-by-step protocol and tips for immunohistochemistry on paraffin-embedded sections. Download PDF version Preparing Fixed Tissues for Paraffin-Embedded Sectioning Here we describe the procedure for preparing formalin-fixed paraffin-embedded tissue (FFPE) samples. ...