结果显示PD-L1阳性IHC染色的患者数(37.5%-57.5%),在去糖基化后H得分(H-SCORE 即histochemistry score (组织化学评分),以下简称H得分)显着增加2倍以上,表明PD-L1的N-糖基化对其识别产生严重影响。 图4. 去糖基化显著增强人肿瘤组织样本中PD-L1的检...
结果显示PD-L1阳性IHC染色的患者数(37.5%-57.5%),在去糖基化后H得分(H-SCORE 即histochemistry score (组织化学评分),以下简称H得分)显着增加2倍以上,表明PD-L1的N-糖基化对其识别产生严重影响。 图4. 去糖基化显著增强人肿瘤组织样本中PD-L1的检测 3、去糖基化后PD-L1 检测的改善与PD-1/PD-L1 治疗...
7号染色体上显示MET与EGFR、CDK6或BRAF的相对位置,用于确定基于NGS的MET局灶扩增。(C)通过免疫组化(IHC)分析C -MET过表达状态的代表性免疫组化图像为阴性(H-score<200)和阳性(H-score≥200)。(D)Venn图显示了FISH、NGS和IHC检测METamp的一致性。(E) NGS和IHC与FISHMETamp的一致性。 NGS与FISH的不一致主要...
由结果可得，该IHC图的阳性细胞百分比为19.739%，阳性细胞百分比≤25%，计1分。综合，该IHC图的综合IHC score得分为：1（细胞染色强度）*1（阳性细胞百分比）=1分。参考文献 [1] Meng J, Liu Y, Han J, Tan Q, Chen S, Qiao K, Zhou H, Sun T, Yang C. Hsp90β promoted endothelial ...
Validation of Automated H-Score Wholeslide Analysis of CEA-Expressing Tumor Cells in IHC Brightfield ImagingLorsakul, AuranuchYao NieAndersson, EmiliaKlaman, IrinaJournal of Pathology Informatics
An H-score of more than 1 is positive. PgR was quantified by the percentage of cells staining positive, and this was divided by 10 to obtain a variable between 0 and 10 (PgR10). HER2 assessment was given as either a positive (IHC3 þ or IHC2 þ and CISH or FISH positive) or ...
c Graph of STRAT4 PGR dCt values by PR IHC H-Score. H-Score is defined as [3(% of tumor staining 3+)] + [2(% of tumor staining 2+)] + [1(% of tumor staining 1+)]. Quantitative stratification of the IHC protein assessment by combining percentage of immunostained tumor...
Indeed, Goldstein et al10 have demonstrated that at least 6–8 h of formalin fixation time for breast biopsies is required to obtain reliable ER determination by IHC; pathologists therefore risk false-negative ER studies when tissues are under- fixed, as can happen in a specimen that comes in...
H&E... AA Salem,AG Douglas-Jones,HM Sweetland,... - 《European Journal of Surgical Oncology》 被引量: 89发表: 2006年 Immunohistochemical assessment of hormone receptor status using a new scoring system (J-score) in breast cancer The assessment of hormone receptor status in breast cancer is...
ER was quantified by using the H-score, which is defined as the percentage of cell staining weakly plus 2 × the percentage of cell staining moderately, plus 3 × the percentage of cell staining strongly, and was considered positive if >1. The variable ER10 was derived by dividing the H...