右击，选择Group alignments by，选择first-in-pair strand 上图这两个基因因为转录方向不同，所以First in pair的这条reads比对到基因组上的方向也不同。向右转录（👉）的基因，First in pair比对到负链上，向左转录（👈）的基因，First in pair比对到正链上。
By default, counting is combined among both strands. This setting outputs the count for each strand separately. Legal argument values are 'read' or 'first'. 'read' Separates count by 'read' strand, 'first' uses the first in pair strand". Results are saved in a separate column for .wig ...
右击,选择Group alignments by,选择first-in-pair strand igv_snapshot.png 上图这两个基因因为转录方向不同,所以First in pair的这条reads比对到基因组上的方向也不同。向右转录(👉)的基因,First in pair比对到负链上,向左转录(👈)的基因,First in pair比对到正链上。 说白了,链特异性建库会告诉你,测...
发现:双末端测序中两条reads的颜色只是为了区分在基因组中位置的先后,蓝色并不代表Second in pair,红色并不代表First in pair。红色表示比对到了DNA双链中上面这条链,即正链,注意不是正义链,蓝色表示比对到了DNA双链中下面这条链,即负链 从右向左转录的基因,First in pair往往是左面这条reads,Second in pair...
I took a trip to Shanghai with my mother during the seven-day holiday. It took us more than two hours to drive to Shanghai from my home in Haimen. We stayed in a large hotel on the eighth floor. On the first day, I just stayed in the hotel and rested. On the second day, my...
Should I have kids first, or a career first?" 还是以后再说?我是该先要孩子,还是先顾事业? All of these are consuming questions. And they're going to answer these questions, whether or not it means not doing all the work I assign and not getting a good grade in my courses. 这些都是...
The bosses each have a through bore for\nreceiving the screws comprising in sequence a\nfrustoconical surface, a first cylindrical surface having\na diameter slightly larger than the crest diameter of the\nmounting screw threads, and a second cylindrical surface\nhaving a diamete...
colorBy | Alignment color option: one of "none", "strand", "firstOfPairStrand", "pairOrientation", "tlen", "unexpectedPair", or "tag". If colorBy = "tag" specify tag with colorByTag. ***NOTE*** As of release 3.0 color by tag is specified as 'tag:<tag name>' | "unexpectedPa...
Well, alignments with snps also disagree with the reference. I'm not happy with it but as a working name I called it "group by > structural variant evidence". Hopefully I come up with something better before release. I added "group by" first because its trivial to do. By coincidence I...
information file to corresponding names in the data files. If the names match this section can be omitted. The format is two-column tab delimited. The first column is the sample name in the data file; the second column is the sample identifier in the attributes information. Example: `...