计数分泌小鼠IgG以及抗原特异性小鼠IgG的B细胞。 试剂盒含有捕获抗体,检测抗体,链霉亲和素-ALP(HRP)和多克隆激活剂。 适用于想要设置和优化实验的客户。 高度敏感,稳定且易于标准化。 Tips: 抗原特异性B细胞有两种可供选择的方式计数:在检测步骤中通过用抗原包被或通过使用生物素化的抗原。对于记忆B细胞,我们建议用...
抗体与细菌或其他颗粒性抗原结合后,通过Fc段与吞噬细胞表面的受体结合,促进吞噬细胞的吞噬作用。 Antibody dependent cell mediated cytotoxicity,ADCC ADCC抗体依赖性细胞介导的细胞毒作用ADCC:抗体与肿瘤细胞、病毒感染细胞表面的抗原结合,通过FC段与NK细胞表面相应受体结合,可触发对靶细...
近年来,单个B细胞抗体筛选技术逐渐发展起来,进而被应用于单克隆抗体的制备,该技术通过流式细胞分选的方法,将特异性抗原进行荧光标记,利用抗原可以特异性结合B细胞表面BCR的原理,将单个目的B细胞筛选出来,用PCR扩增的方法得到抗体的重链和轻链基因,将抗体基因构建在表达载体上,转染到真核细胞中表达出单克隆抗体(图1),...
3.1 B细胞清除治疗(B cell depletion therapies,BCDT) BCDT是利用抗体药物靶向结合B细胞表面特异性抗原,通过抗体依赖细胞介导的细胞毒性作用(antibody-dependent cell-mediated cytotoxicity,ADCC)或补体依赖的细胞毒性作用(complement dependent cytotoxicity,CDC)清除B细胞。也可靶向...
本文主要是简单的说明IgX抗体,X是什么意思、来源和历史,具体请阅读参考文献的论文,由于生命科学的特殊性,本文不能当作权威材料,仅供饭后娱乐。 IgG:IgG血清中抗感染免疫的主要抗体,约占血清中抗体总量的75%。是5类抗体中唯一可以通过胎盘的抗体。 胎儿出生3月后,合成IgG, 2-3岁时达到成人量。 G是gamma-globuli...
(termed IgGE). Mucosal grass pollen allergen exposure by SLIT resulted in highly diverse IgE and IgGE repertoires. These were extensively mutated and appeared relative stable as per heavy chain isotype, somatic hypermutations and clonal composition. Single IgGE + memory B cell and IgE+ pre-...
CHEN Yang, LIU Tong, ZHANG Jia-qi, LIAO Hua-xin, LIN Yue-zhi, WANG Xiao-jun, WANG Ya-yu. Screening of Monoclonal Antibodies Targeting the Equine IgG1 Based on Single B Cell Antibodies Gene Amplification Technology. Journal of Chinese Biotechnology, 2022, 42(4): 17-23 doi:10.13523/j.cb...
B cells and the antibodies they produce are critical in host defense against pathogens and contribute to various immune-mediated diseases. B cells responding to activating signals in vitro release extracellular vesicles (EV) that carry surface antibodies, yet B cell production of EVs that express ...
3.1 B细胞清除治疗(B cell depletion therapies,BCDT) BCDT是利用抗体药物靶向结合B细胞表面特异性抗原,通过抗体依赖细胞介导的细胞毒性作用(antibody-dependent cell-mediated cytotoxicity,ADCC)或补体依赖的细胞毒性作用(complement dependent cytotoxicity,CDC)清除B细胞。也可靶向作用于B细胞存活所需要的细胞因子诱导B细...
b, and Supplementary Fig.9afor gating strategy, T cell only and non-transferred controls). In all memory B and T cell recipient mice, there was a large population of endogenous B220+CD138−B cell precursors, a small population of donor-derived B220+IgG1+cells, and very few B220+IgE...