N = 3 repetitions for cell lines. Transwell assay Cell migration and invasion were determined using Transwell chambers (Corning Costar, diameter = 8 μm). The single-cell suspension (1 × 105; 300 μl) diluted in serum-free medium was added to the upper chamber (with ...
Cell proliferation was scored by assessing the percentage of cells positive for Ki67 nuclear antigen in 100 epithelial cells for vehicle-exposed endometrium in the proliferative phase (vehicle proliferative phase, n ¼ 7), secretory phase (vehicle secretory phase, n ¼ 7) of the cycle and the...
diameter = 8 μm). The single-cell suspension (1 × 105; 300 μl) diluted in serum-free medium was added to the upper chamber (with or without matrix glue), whereas the basolateral chambers were filled with 10% FBS-supplemented medium. The cells were allowed to migrate fo...
cell reprogrammingIGFI/AKT/mTOR signalingribosomal proteincell cycleThe generation of induced pturipotent stem cells (iPSCs)offers a great opportunity in research and regenerative medicine.The current poor efficiency and incomplete mechanistic understanding of the reprogramming process hamper the clinical ...
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Primary cell culture and tumor sphere formation assay Patient-derived tumor tissues were treated according to a previously described method.16 We previously confirmed that patient-derived tumor cells plated at 5000 cells per ml yield tumor spheres that are clonally derived from single cells.16 Sphere...
We also evaluated the exact methylation level of each m6A site in the CDS sequence of PD-L1 mRNA at a single-nucleotide resolution using a new method for absolute quantification of m6A levels. Additionally, METTL3 knockdown could functionally improve T-cell killing and inhibit T-cell exhaustion ...
(G) mRNA expressions of IGF2BP1 were detected from two normal colon epithelial cell lines and five colon cancer cell lines. (H) LoVo cells were transfected with control vector or IGF2BP1 overexpression vector for 48 h, followed by exposing to normothermia or HT. (I and J) Cell survival...
Independent hESCs (H1, H9, Shef6) and iPSCs (RCiB10) adapted to AI medium have been propagated for multiple passages, including lines that have been maintained for over 24 months and over 70 passages. Cells were passaged on an approximate 4 to 5-day cycle using Accumax single-cell dissoci...
We then sorted patient-derived primary breast cancer cells using the anti-IGF1 R antibody to obtain cell populations in which IGF1 R was expressed at either high or low levels. When we stimulated these cells with IGF2 or HRG for sphere formation, the IGF-1Rhigh cell population gave rise ...