Single-cell imaging flow cytometry was used to determine nuclearization of Smad3 and intracellular fluorescence intensity of Smad7 and the corneal crystallin aldehyde dehydrogenase 3A1. Extracellular matrix proteoglycans keratocan and biglycan were quantified using ELISAs. On the TGF-β2 background, the...
N = 3 repetitions for cell lines. Transwell assay Cell migration and invasion were determined using Transwell chambers (Corning Costar, diameter = 8 μm). The single-cell suspension (1 × 105; 300 μl) diluted in serum-free medium was added to the upper chamber (with ...
Cell Death & Disease (2023) The neurobiology of insulin-like growth factor I: From neuroprotection to modulation of brain states A. Nuñez J. Zegarra-Valdivia I. Torres Aleman Molecular Psychiatry (2023) IGF-1 Combined with OPN Promotes Neuronal Axon Growth in Vitro Through the IGF-1R/Akt...
The average area of a single quadriceps muscle cell in Qui-treated mice was larger than that in CR- or AC-treated mice (Fig. S2E). SLAs elevate hepatic IGF-1 in piglets and mice Postnatal growth is regulated by activities of the somatotropic axis [30, 31], where GH secreted by the ...
(2008). Insulin-like growth factor 2 is required for progression to Oncogene 3646 Igf2 dependent Pten þ /À phenotypes DN Church et al advanced medulloblastoma in patched1 heterozygous mice. Cancer Res 68: 8788–8795. Crackower MA. (2002). Regulation of myocardial contractility and cell ...
For both living and fixed cells, Raman imaging serves as an alternative. While acknowledging the heterogeneity of leukemic cell types and normal white blood cells, and the variety of sample preparation methods employed, this work sought to demonstrate the efficacy of these techniques in Raman ...
A Randomised Trial Evaluating the Safety and Efficacy of a Single Low Dose of Intracoronary Insulin-like Growth Factor-1 Following Percutaneous Coronary Intervention for ST-Elevation Acute Myocardial Infarction (RESUS-AMI) When a patient has a heart attack, a blockage occurs in a coronary artery tha...
(99m)Tc-CCND1-Peptide nucleic acid (PNA)-IGF1 peptide chimeras ((99m)Tc-WT4185) is a (99m)Tc-peptide-PNA-peptide chimera that was developed as a gene expression agent for gamma planar/single photon emission computed tomography (SPECT) imaging of breast cancer (1, 2). (99m)Tc-WT418...
The major function of Insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2) is to regulate cell metabolism. However, emerging evidence indicates that IGF2BP2 plays a role in cancer, but the underlying mechanism is largely unknown. Here we showed that upregulation of IGF2BP2 is ass...
Recent studies have suggested that mRNA internal m7G and its writer protein METTL1 are closely related to cell metabolism and cancer regulation. Here, we identify that IGF2BP family proteins IGF2BP1-3 can preferentially bind internal mRNA m7G. Such inter