To test this, we used a luciferase reporter assay in HEK293T cells to probe the impact of HNF4A on the IFN尾 promoter, which is controlled by IRF3/7. HNF4A potently suppressed IFN尾 promoter activity following viral stimulation. HNF4A DNA-binding activity is necessary for this suppression...
1000 IU/ml of recombinant IFN-β were added. After 24 h of incubation, the relative luciferase activities of IFN-treated cells were determined. This result illustrates the induction of a luciferase reporter gene under control of