The lysates were cleared by centrifugation (12,000 × g for 30 min at 4 °C) before proceeding with purification as described in details below for the different proteins. In all cases the proteins of interest were expressed fused with a N-terminal His6SUMO-tag, which was removed ...
Answer to: A buffer solution is made using a weak acid, HA, that has a pKa of 5. If the pH of the buffer is 7, what is the ratio of A- to HA? By...
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Since the granules are often positioned in close distances and their diameter is often below 300 nm, a high-resolution microscopy technique has to be employed to estimate their amount in the cells. We used structured illumination microscopy (SIM) followed by Amira software analysis of insulin ...
Although the physiological pH of human arterial blood is between 7.35 and 7.45, acidosis is said to occur when arterial pH falls <7.35 and alkalosis when arterial pH is >7.45. To calculate the pH of blood, the standard Henderson–Hasselbalch equation can be used:pH=pKa+log([HCO3−][H2CO...
The Henderson-Hasselbalch equation is given below: {eq}\begin{align*} \text{pH} &= \text{pKa} + \log\frac{[\text{A}^-]}{[\text{HA}... Learn more about this topic: How To Calculate the PH of a Buffer Solution | Equation ...
A buffer solution is made using a weak acid, HA, that has a pka of 6. If the pH of the buffer is 5, what is the ratio of A to HA? A buffer solution is made using a weak acid, HA, that has a pKa of 5. If...
Answer to: A buffer solution is made using a weak acid, HA, that has a pKa of 6. If the pH of the buffer is 4, what is the ratio of A- to HA? By...
Simultaneously, a mixture of 30.24 g piperazine-N, N’-bis (2-ethanesulfonic acid) (PIPES), 15 g agar, 900 mL distilled water, and 50% (w/w) NaOH, where the pKa was adjusted to 6.8 with PIPES, was also autoclaved. Finally, the two mixtures were combined and gently agitated to ...