Although carboxyfluorescein is better retained in cells than is fluorescein, its pKa of ~6.5 is lower than the cytosolic pH of most cells (pH ~6.8–7.4). Consequently, its fluorescence change is less than optimal for detecting small pH changes above pH 7. Since its intr...
Although carboxyfluorescein is better retained in cells than is fluorescein, its pKa of ~6.5 is lower than the cytosolic pH of most cells (pH ~6.8–7.4). Consequently, its fluorescence change is less than optimal for detecting small pH changes above pH 7. Since its introd...
This can be seen as an advantage but means that great care must be taken over the interpretation of the parameters. The derivation also shows that a = m and b = − xm, so that it is possible to obtain an estimate of the average exchange ratio from Eq. [9], i.e., x = − ...
This means the relationship between the migration time and pI is not linear. The loss of linearity is a consequence of mobilization, not of focusing. Sign in to download full-size image FIGURE 5.12. The pH gradient at different mobilization times on a 2-mm slab gel (3%C, 6%T) cast in...
AHAs are less oil soluble than BHAs, so they supposedly can’t penetrate clogged pores as well, but AHAs have an additional moisturising effect. 窗体底端 3 AHAs and BHAs are acids, which means they can donate H+ions, the things that make acids acidic. For glycolic acid, it looks like...
Salicylic acid is a larger molecule than most AHAs. This makes it quite oily – oily enough that having a little charge on the end might not make it all that less oily. This means that it can still work effectively at a higher pH, as demonstrated in these two studies. (I wasn’t aw...
migrating single cells create their own localized pH nanodomains at their surface and in the cytosol. By this means, the migrating cells locally modulate their adhesion to, and the re-arrangement and digestion of, the extracellular matrix. At the same time, the cytosolic nanodomains tune cytoskelet...
Error bars represent the SD of means of independent replicates (n = 3). c Analysis of low pH-triggered conformational changes in fusion-deficient GP64 constructs. At 36 h p.t., the cell-surface-localized GP64 proteins subjected to low pH triggering were measured by cELISA using MAb...
The pH of milk of magnesia is about 10.5. The pH scale ranges from 0 to 14. Solutions with a pH less than 7 are considered acids, and solutions with...Become a member and unlock all Study Answers Start today. Try it now Create an account Ask a question Our experts can answer ...
The phosphate buffer system has a pKa of 6.8, which means that there are similar concentrations of H2PO4− and HPO4− at the pH of 7.40. Therefore, the phosphate buffering system has its best buffering capacity in the normal blood pH range. Yet, the concentrations of H2PO4− and HP...