Our objective was to optimize the iDISCO protocol for use in porcine intestinal tissue to allow complete qualitative and quantitative analysis of postnatal development of the enteric glial network in our comparative pig model. Antibodies against glial cell markers S100b, Sox10, and glial fibrillary ...
14. 随后在100% DCM中孵育2 × 15 min以洗涤甲醇。 15. 最后,样品在二苄醚(DBE)中孵育,直到透明化(约4 h),即可成像。 Protocal下载地址 whole-mount-staining-bench-protocol-methanol-dec-2016idiscodotinfo.files.wordpress.com/2015/04/whole-mount-staining-bench-protocol-methanol-dec-2016.pdf 关于...
We first tested antibodies on sections pretreated with methanol to mimic the effect of methanol in the iDISCO protocol. Twenty-five of the antibodies gave good results in that test (Table S3) and all performed very well in iDISCO using methanol pretreatment (i.e., with similar signal-to-...