14. 随后在100% DCM中孵育2 × 15 min以洗涤甲醇。 15. 最后,样品在二苄醚(DBE)中孵育,直到透明化(约4 h),即可成像。 Protocal下载地址 whole-mount-staining-bench-protocol-methanol-dec-2016idiscodotinfo.files.wordpress.com/2015/04/whole-mount-staining-bench-protocol-methanol-dec-2016.pdf 关于...
Antibodies against all three markers tested produced the predicted staining pattern with minimal non-specific staining. Percent of jejunal villus volume occupied by GFAP+ glia is higher in 6-week-old versus 2-week-old pig (0.49% versus 0.23%, *P鈮 0.05). Ongoing work will optimize ...
Various Thy1::GFP lines were developed as a modern alternative to Golgi staining to label excitatory projection neurons (Feng et al., 2000). Using an Abcam anti-GFP antibody (Table S3), we tested iDISCO on the Thy1::GFP-M line, which has the most sparse GFP expression of all reported...