The method examines polymorphisms of a (dT-dG)n VNTR region at the D11S35 locus on human chromosome 11. The method comprises the steps of isolating the human DNA, heat-denaturing the DNA to form single strands, hybridizing synthetic DNA oligomers as described herein to the denatured DNA so ...
The DNA damage response serine/threonine kinase ATM was also found to be essential for iron metabolism. ATM induced ferroptosis by decreasing the expression of iron regulators involved in iron storage (ferritin heavy and light chain, FTH1 and FTL) and export (ferroportin, FPN1), moreover, an ...
Specifically, using bioinformatics tools, we detected the precise insertion locations and copy numbers of transfer DNA, genetic rearrangements, and the absence of backbone sequences, which were equivalent to results obtained from Southern blot analyses. NGS methods have been suggested as an effective ...
Understanding the difference in genetic regulation of gene expression between brain and blood is important for discovering genes for brain-related traits and disorders. Here, we estimate the correlation of genetic effects at the top-associatedcis-expression or -DNA methylation (DNAm) quantitative trait...
The method comprises DNA extraction from the sample; amplification of divergent segments of the cytoplasmatic beta-actin gene by PCR or an equivalent technique, using primers of regions with high evolutionary conservation between species and subspecies; and identification of the amplified segment by ...
The discovery of cell-free fetal DNA (cffDNA) in the maternal blood circulation of pregnant women [5] in the 1990's provided with a novel approach to target fetal genetic material during pregnancy. Research carried out over the last three decades has shown that individual variations [6], sta...
The WM-800 population and the founders and checks were genetically characterized with the Infinium 15 K iSelect SNP array and the 135k Affymetrix SNP array at TraitGenetics (Gatersleben, Germany) using bulked DNA from 12 F4:5 seedlings per WM-800 line and founder. In total, 27,006 pol...
However, morphological descriptions can possess limitations, as morphology can be influenced by environmental factors and requires skilled assessment7. With the advent of molecular marker techniques, DNA fingerprinting has become an important tool used to identify and delineate cultivars and quantify ...
The heterogeneity of Parkinson’s disease (PD) has been recognized in clinical, with patients categorized into distinct subsets based on motor phenotype, such as tremor-dominant PD (TD), postural instability and gait difficulty-dominant PD (PIGD) and mixed PD (Mix). Despite this categorization,...
"Transformed host" refers to a cell into which (or into an ancestor of which) a DNA molecule has been introduced by means of recombinant DNA techniques. "High stringency conditions" refers to hybridization conditions used to identify nucleic acids that have a high degree of homology to the pro...