Acute myocardial infarction (AMI) has two clinical characteristics: high missed diagnosis and dysfunction of leukocytes. Transcriptional RNA on leukocytes is closely related to the course evolution of AMI patients. We hypothesized that transcriptional RN
In contrast to a potency screen or primary pharmacology carried out in the earliest (discovery) phases of drug research, a secondary pharmacology screen, performed when drug candidates reach the translational phase (e.g., preclinical safety), is required to cover a broad array of target classes,...
Lung adenocarcinoma (LUAD) is the predominant histological subtype of lung cancer and the leading cause of cancer-related mortality. Identifying effective drug targets is crucial for advancing LUAD treatment strategies. This study employed proteome-wide
we demonstrated that depletion of the nicotinamide adenine dinucleotide synthetase (NadE) rapidly killed Mycobacterium tuberculosis under conditions of standard growth and nonreplicative persistence induced by oxygen and nutrient limitation as well as during the acute and chronic phases of infection in mice...
we conceived a computational workflow that included independent in vitro validation, followed by assessing emerging candidates in the context of available clinical pharmacology data with the aim of proposing suitable candidates forclinical studiesfor early stage (incubation and symptomatic phases) patients in...
The genomic analysis was first used to identify genomic differences in the effect of SARS-CoV-2 on Homosapiens. This genomic level study will allow researchers to focus on SARS-CoV-2 and the major risk factors in the future. 1.1. Materials and methods There are seven major phases in this ...
Fig. 1: All-over workflow of the study. The study included three phases. Phase 0 consisted of discovery proteomics by untargeted mass spectrometry to identify putative biomarkers, followed by phase I in which targets from the discovery phase were transferred to a targeted, mass spectrometric MRM ...
RNA polymerase is essential to the viability of bacteria in all phases of growth and development and is a proven chemotherapeutic target as the cellular target of the rifamycin class of antibiotics. However, despite the characterization of multiple different classes of natural products that selectively...
So the selection for above validation datasets met the requirements of MR analysis and ensured these data were suitable to be employed in further replicative phases. The cis-pQTL filtering process was identical to that of the discovery phase. and sensitivity analyses (heterogeneity test, pleiotropy ...
cDNA for the predicted zebrafish coPARSE-lncRNA homologs were amplified from zebrafish mixture cDNA samples of different developmental phases (gift from A.M. Meng laboratory, Tsinghua University) or synthesized by TsingKe Biotech. For rescue plasmids, homologs amplified by PCR were inserted into AvrII...