完整的试剂盒包含用于细胞裂解、染色质剪切、免疫沉淀和 DNA 纯化的所有缓冲液和试剂。此外,与竞争解决方案不同,该试剂盒包含阳性和阴性对照抗体(分别为 H3K4me3 和 IgG)以及阳性和阴性对照PCR引物对(分别为 GAPDH TSS 和肌红蛋白外显子 2),为您提供便利并保证最佳结果结果。该试剂盒已针对多个组蛋白标记进行了...
From a mechanistic standpoint, the lack of significant change in intracellular concentration of Gln when the supply of Leu increased could be a reason for the lack of response on mTORC1. Previous data indicated that by promoting the influx of Leu through a bidirectional transport mechanism, Gln ...
For the DNA extracted from heart FFPE tissue, the DNA concentration was below the quantitation range of the Genomic DNA ScreenTape assay (10 ng/µL) and the majority of samples were below the functional quantitative range for DIN (5 ng/µL). For DNA concentrations below 3 ng/µL,...
RT-PCR analysis Total cellular RNA was isolated using an RNeasy mini kit (Qiagen, Venlo, Limburg, Netherlands). The RNA samples were treated with DNA-free DNase I (Ambion, Life Technologies - Carlsbad, CA, USA), according to the manufacturer’s instructions and reverse-transcribed into cDNA ...
Hence, an exploration of ideal sources of variability to high temperature tolerance for wheat and other field crops is required. High temperature fluctuations have a significant impact on plant development in a variety of ways. Chlorophyll concentration and the photosynthetic ability of leaves are ...
The method using Monilinia-specific DNA primers of the internal transcribed spacer (ITS region) was employed by Forster and Adaskaveg [90] in the detection of early brown rot infections in cherry fruits. With the same technique (ITS), Ioos and Frey [91] used the endpoint PCR to study ...