Antibody Refs Type WB EL ICC IP IHC FC LSBio 49 antibodies antibodies-online 157 antibodies GeneTex 6 antibodies Novus Biologicals 27 antibodies Invitrogen Antibodies 29 antibodies OriGene 22 antibodies Biorbyt 81 antibodies Cell Signaling Technology, Inc ...
Cell lysates (0.5–1 mg of protein) were mixed with anti-Flag antibody and protein A/G beads and incubated for 3 h at 4 °C with gentle agitation. The beads were then washed in deacetylase buffer (50 mM Tris-HCl, pH 9.0, 4 mM MgCl2, 0.2 mM DTT) and resuspended ...
Applications Tested: This DANID2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 1.0 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume...
Antibody 克隆号 DANID2 抗原 Mammalian cell line expressing the full form of CD186 查看免疫原 偶联物 PE Request custom conjugation 激发/发射光谱 565/576 nm查看光谱 形式 Liquid 浓度 0.2 mg/mL 纯化类型 Affinity chromatography 保存液 PBS, pH 7.2 ...
保存条件 :Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody...
Tumors were excised, mechanically minced, incubated in collagenase solutions for 20 min at 37 °C, and then filtered to generate a single-cell suspension. After filtration, the cells were subjected to antibody staining at 4 °C for 20 min in RPMI 1640 medium and sorted by flow cyt...
Experimental Design: Antibody array-based screening was used to identify differentially secreted growth factors from Id1-overexpressing esophageal cancer cells. In vitro and in vivo assays were performed to confirm the induction of IGF2 by Id1, and to study the autocrine and endocrine effects of IG...
Open AccessArticle by Jumpei Ito 1,†, Tomomi Minemura 1, Sébastien Wälchli 2, Tomoaki Niimi 1, Yoshitaka Fujihara 3, Shun’ichi Kuroda 4, Koichi Takimoto 5and Andrés D. Maturana 1,* 1 Laboratory of Animal Cell Physiology, Graduate School of Bioagricultural Sciences, Nagoya University,...
5B). The antibody used in these experiments was pan-methyl H3K9 that recognizes all three states of H3K9 methylation. We next examined the methylation of H3K27 as JARID2 has been shown to direct the PRC2 complex to target genes (31, 32) and found that depletion of JARID2 led to a ...
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