DYKDDDDK Tag Antibody (HRP Conjugate) detects exogenously expressed DYKDDDDK proteins in cells. The antibody recognizes the DYKDDDDK peptide (the same epitope recognized by Sigma's Anti-FLAG® antibodies) fused to either the amino or carboxy te...
DYKDDDDK Tag (D6W5B)Rabbit mAb detects exogenously expressed DYKDDDDK proteins in cells. The antibody recognizes the DYKDDDDK peptide, which is the same epitope recognized by Sigma-Aldrich Anti-FLAG M2 antibody, fused to either the amino-...
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FLAG is an affinity tag widely used for rapid and highly specific one-step protein purification. Native elution of protein from anti-FLAG antibody resins allows the identification of protein and nucleic acid binding partners and functional analysis using biochemical activity assays.Access...
产品名称:anti-DYKDDDDKTag(NT)/Flag-Tagpolyclonalantibody??抗标签DYKDDDDKTag抗体(N端) 品牌:白益 货号:BY-K0833R 详见说明书。 单克隆抗体: 解决多克隆抗体特不高的理想方法是制备识别单一表位特的抗体。如果能获得仅针对单一表位的浆细胞克隆,并使其在体外扩增分泌抗体,就有可能获得单一表位特的抗体。然而,...
200 [micro]l of the quantified lysates was incubated with 1 fig of mouse anti-Flag M2 (Sigma-Aldrich, F1804) or anti-HA tag (C29F4) antibody (Cell Signaling Technology, number 3956) at 4[degrees]C for overnight incubation. Critical Role of HAX-1 in Promoting Avian Influenza Virus Repl...
antibodies. A FLAG-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization ...
expression and purification.1-3 The ANTI-FLAG ® M1 Agarose Affinity Gel is a covalent conjugate of a purified mouse IgG2B monoclonal antibody to agarose by a hydrazide linkage. The ANTI-FLAG ® M1 antibody has a binding specificity for FLAG ®-tagged proteins with the FLAG ®-tag at...
ANTI-FLAG® antibody, Rat monoclonal clone 6F7, purified from hybridoma cell culture; Synonyms: Anti-ddddk,Anti-dykddddk; find Sigma-Aldrich-SAB4200071 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich
permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Eac...