Additionally, REV1, Pol味, and the FANCD2/FANCI heterodimer have been shown individually to be involved in homologous recombination (HR) repair, which is crucial for resolution of DNA DSBs and ICL repair during S phase. We demonstrate that human REV1 and REV3 exhibit an epistatic relationship ...
DNA interstrand crosslinks (ICLs) are extremely deleterious lesions that are repaired by homologous recombination (HR) through coordination of Fanconi anemia (FA) proteins and breast cancer susceptibility gene 1 (BRCA1) product, but the exact role these proteins have remains unclear. Here we report ...
DNA INTERSTRAND CROSSLINK REPAIR IN MAMMALIAN CELLS: STEP BY STEP Work in E. coli identified a two cycle repair scheme involving incisions on one strand on either side of the ICL (unhooking) producing a gapped ... PA Muniandy,J Liu,A Majumdar,... - 《Critical Reviews in Biochemistry & ...
Because DpnII has a restricted number of sites across the genome, this method has limited coverage of captured RNA–DNA interactions9. In addition, the technology does not size select the molecules containing both interacting RNA and DNA, resulting in a large fraction of uninformative sequences in...
(6)关节滑膜细胞分泌的细胞因子(IL-1、IL-2、TNF-a)可能在OA防病中起着重要作用,其他可干扰软骨糖蛋白合成,加速纤维细胞合成,使基质分子下降;(7)病变关节软骨自身免疫反应能力增强,软骨损伤成分暴露,引起抗自体软骨成分的自身免疫反应,产生的抗胶原体可抑制软骨细胞的DNA、硫酸多糖和胶原的合成,加重软骨损伤,形成...
由可重构的A-motif双链、i-Motif四链和T-A-T三链结构介导的pH级联DNA水凝胶; 三维打印用于高效软骨和软骨下骨再生的微环境特异性生物启发和外泌体强化水凝胶支架; 用于骨软骨缺损双线再生的镁梯度分层支架; 包裹血管瘤干细胞衍生细胞外囊泡的仿生热敏水凝胶促进糖尿病伤口的微循环重建; ...
Genetic interactions of the Saccharomyces cerevisiae DNA Repair Factor PSO2 DNA interstrand cross-links (ICLs) present a major challenge to cells, covalently binding the two DNA strands together and preventing fundamental cellular ... Ward,T Anthony - Oxford Brookes University 被引量: 0发表: 2013年...
The Fanconi Anaemia Pathway Promotes Replication-Dependant DNA ICL repair.Knipscheer, Puck
The main goals of this proposal are: 1) to develop and optimize a rapid method to assess the ability of breast tumor cell lines to repair specifically DNA interstrand crosslinks (ICLs). 2) to apply this technology to a set of human breast cancer cell lines. In addition, we propose to ...
Eledtroporatiom off mormal tamami DNA eedomiiiiclleases into ceis corrects tlheir EDNA repair defectincrease UDS above background levels. In contrast, electro- poration of endonucleases, pi 4.6 or 7.6, obtained from XPA cells into psoralen plus UVA tr...