Iba1-immunopositive cells in VH, Cx and Th of saline- and LPS-injected rats.Furong, ZhuYingjun, ZhengYuQiang, DingYong, LiuXianghui, ZhangRenrong, WuXiaofeng, GuoJingping, Zhao
Immunofluorescence analysis of IBA1 was performed using 70% confluent log phase THP-1 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled...
Positive IP detected in THP-1 cells Positive IHC detected in rat brain tissue, human liver tissue, human tonsillitis tissue, human brain tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate bu...
Flow cytometric analysis of fixed/permeabilized SH-SY5Y cells (blue, negative) and THP-1 cells (green, positive) using Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (Pacific Blue™ Conjugate) (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control (Pa...
Positive FC (Intra) detected in THP-1 cells Recommended dilution ApplicationDilution Immunoprecipitation (IP) IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC) IHC : 1:400-1:1600 Immunofluorescence (IF)-P IF-P : 1:50-1:500 Flow Cytometry (FC) (INTRA) FC...
靶标信息 AIF1 is induced by cytokines and interferon. It is thought to be involved in the negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. 仅用于科研。不用于诊断过程。未经明确授权不得转售。相关...
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Positive samplesRat spleen Cellular locationCell projection, Cytoplasm, Cytoplasmic side, Peripheral membrane protein, cytoskeleton, ruffle membrane Customer validation- About the Immunogen ImmunogenRecombinant fusion protein of human Iba1 (NP_001614.3). ...
In agreement with expression patterns of CD68+ and CD163+ macrophages, cells reacting to Iba1 and Gal-3 were increased in numbers on post-injection (PI) days 1–5, peaking on day 2; thereafter, the positive cells gradually decreased to control levels until PI days 7 and 10. The ...
Kupffer cells are positive for Iba-1. See Abreview Western blot - Anti-Iba1 antibody (ab107159) Anti-Iba1 antibody (ab107159) at 1 µg/ml + Rat Cortex Tissue Lysate at 10 µgSecondaryRabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using ...