7.体外3-D培养人细胞间质干细胞(hMSC)进行骨诱导 “Gene expression profile of human mesenchymal stem cells during osteogenesis in three-dimensional thermoreversible gelation polymer”Biochem. Biophys. Res.Commun.,317, 1103-1107 (2004). 8.人类肝细胞3-D培养生产丙型肝炎病毒(HCV) “Production of infec...
The microglial cells displayed a homogenously distributed Iba1—staining pattern in the aMCC with slightly varying activation states in all three groups. The estimated microglial densities did not differ significantly between individuals with schizophrenia, BD and control subjects. Remarkably, when both ...
→使用抗Iba1,单克隆抗体(Wako产品编号:012-26723)小胶质细胞的定位信号清晰,与抗Iba1,兔多克隆抗体的效果相同(Wako产品编号:019-19741 019-19741)。 ◆实验条件 ● 样品:7周龄大鼠,7周龄小鼠或成年狨猴的大脑皮质 ●切片:50μm冰冻切片(小鼠和大鼠),40μm冰冻切片(狨猴) ●染色法:ABC法+ DAB染色 ●抗体...
Anti Iba1, Rabbit (for Immunocytochemistry) Iba1(Ionized calcium-binding adapter molecule 1)是分子量约为17 kDa的钙结合蛋白。由于在中枢神经系统中,Iba1在小胶质细胞中特异性表达1),因此被用作小胶质细胞标志物。据报告称,Iba1在静息型小胶质细胞和活化型小胶质细胞中均有表达,但有报告称在活化型小胶质细...
Calcium ions are known to be one of the most important signal mediators in all cells including central nervous system (CNS) cells. Calcium ions exert their signaling activity through association with various calcium binding proteins, many of which are classified into a large protein family, the EF...
Iba1 is a 17-kDa EF hand protein that is specifically expressed in macrophages/microglia and is upregulated during the activation of these cells. When exposed to macrophage colony-stimulating factor (M-CSF), microglia cell line MG5 immediately produces intense membrane ruffles in which Iba1 accumu...
“Gene expression profile of human mesenchymal stem cells during osteogenesis in three-dimensional thermoreversible gelation polymer”Biochem. Biophys. Res.Commun.,317, 1103-1107 (2004). 8.人类肝细胞3-D培养生产丙型肝炎病毒(HCV) “Production of infectious hepatitis C virus particles in three-dimensiona...
Immunofluorescence analysis of IBA1 was performed using 70% confluent log phase THP-1 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled...
membrane ruffling and RAC activation. It enhances lymphocyte migration and the actin-bundling activity of LCP1. IBA1 also plays a role in RAC signaling and in phagocytosis and involved in vascular inflammation. It also promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes...
Background:Allograft Inflammatory Factor-1 (AIF1)or ionized calcium-binding adaptor molecule 1 (Iba1) is expressed selectively in microglia/macrophages and is a Ca2+-binding peptide produced by activated monocytes and microglial cells. It has been suggested that AIF1 expression is associated with ch...