Immunofluorescence analysis of IBA1 was performed using 70% confluent log phase THP-1 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled...
7.体外3-D培养人细胞间质干细胞(hMSC)进行骨诱导 “Gene expression profile of human mesenchymal stem cells during osteogenesis in three-dimensional thermoreversible gelation polymer”Biochem. Biophys. Res.Commun.,317, 1103-1107 (2004). 8.人类肝细胞3-D培养生产丙型肝炎病毒(HCV) “Production of infec...
Immunofluorescence analysis of IBA1 was performed using 70% confluent log phase THP-1 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled...
Iba1/AIF-1 is also uniquely expressed in cells of monocytic lineage and is, therefore, widely used as a marker for microglia/macrophages in the brain and other tissue (6). Schulze, J.O. et al. (2008) FEBS J 275, 4627-40. Deininger, M.H. et al. (2002) FEBS Lett 514, 115-21...
Background:Allograft Inflammatory Factor-1 (AIF1)or ionized calcium-binding adaptor molecule 1 (Iba1) is expressed selectively in microglia/macrophages and is a Ca2+-binding peptide produced by activated monocytes and microglial cells. It has been suggested that AIF1 expression is associated with ch...
Epithelial Stem Cells from Dermis by a Three-dimensional Culture System”Journal of Cellular Biochemistry, 98 (1),174-184 (2006) 6.体外3-D软骨细胞培养再生软骨组织 Chondrocytes Containing Growth Factors in a Novel Thermoreversible Gelation Polymer Scaffold”Tissue Engineering,12 (5), 1237-1245 (2006...
Flow cytometric analysis of SH-SY5Y cells (blue) and THP-1 cells (green) using Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (PE Conjugate) (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control (PE Conjugate) #57...
Calcium ions are known to be one of the most important signal mediators in all cells including central nervous system (CNS) cells. Calcium ions exert their signaling activity through association with various calcium binding proteins, many of which are classified into a large protein family, the EF...
While the involvement of microglia, immune cells that play an important role in brain inflammation, has recently been suggested not only in neurodegenerative diseases but also in various other neurops… 4th review: Macrophages control the inflammation and subsequent neural repair after ischemic strokeWak...
Human Iba1, His Tag (IB1-H51H9) is expressed from E. coli cells. It contains AA Met 1 - Pro 147 (Accession # P55008-1). Predicted N-terminus: Met 1 蛋白结构(Molecular Characterization) This protein carries a polyhistidine tag at the C-terminus. ...