and increased CD40, iNOS, and Kelch-like ECH-associated protein 1 expression levels, and the number of Iba1-positive cells in the hippocampus, and decreased p-AMPK and LC3II/I expression levels, and the number of NF-E2-related factor 2 (Nrf2)-positive cells, and reduced neurogenesis in th...
a, b, Representative confocal single plane images of CA1 astrocytes (a, S100B, red) or microglia (b, IBA1, red) with synaptic proteins (VGluT1, PSD95, VGAT or Gephyrin, green). White arrows indicate engulfed synaptic proteins in glial cells. Scale bars = 5 μm. c, d, Quantificatio...
Colocalization with Iba1 was performed to determine microglial proliferation. MCM2-positive cells were present in the HC of controls and were significantly increased in the presymptomatic iLBD cases, but not in established PD patients. Microglia represented the majority of the proliferating cells in ...
In contrast, the PE243 isolate induced an increased expression of Iba1 and triggered a morphological transition towards the characteristic phenotype of activated microglial cells, surpassing the control levels of these two parameters at 24 h p.i. and subsequently returning to control levels at 48 h...
Iba1-IR cells were quantified to examine potential differences in the density of microglia in the dentate gyrus. hAPOEε4 rats had a higher density of Iba1-IR cells, regardless of region compared to wildtype rats (main effect of genotype:F[1,22] = 6.898,p = 0.015, partial η...
NEUN+ neurons, IBA1+ microglia, S100B+ astrocytes, and OLIG2+ oligodendrocyte lineage cells were observed. d, Sample confocal images showing EdU-incorporated PROX1+ newborn GCs are absent of the astrocyte marker S100B or the more mature neuron marker CALB1 in slice cultures. Asterisks ...
Regulatory signals from the neurogenic niche direct neural stem cells to proliferate, migrate, extend neurites, form synapses and integrate into existing circuits (Fig. 1b). Dysregulation of any these processes, particularly at the NSC/NPC stage, has the potential to permanently alter neuronal ...
Iba1-IR cells were quantified to examine potential differences in the density of microglia in the dentate gyrus. hAPOEε4 rats had a higher density of Iba1-IR cells, regardless of region compared to wildtype rats (main effect of genotype:F[1,22] = 6.898,p = 0.015, partial η...
Dual immunofluorescence staining for reelin with GAD65, GFAP, Iba1 and Olig2 was performed online using a heat protocol. The double-stainings were performed consecutively and detection for each marker was completed before application of the next antibody. The following primary antibodies were used:...
CA2/3 and DG were delineated at a ×2.5 magnification (Supplementary Fig.1A). The numbers of Iba1+cells in the three subfields of the hippocampus were estimated using the optical fractionator method in the stereological system (Glostrup, Denmark). The total number of Iba1+cells in each hippo...