Identification of Bna.IAA7.C05 as allelic gene for dwarf mutant generated from tissue culture in oilseed rapeSchematic representations of the key components of the transformation vector. . Transgenic elements kanamycin (NPTII) and NosT could not be detected in dwarf mutants. P. positive transgenic ...
Download: Download full-size image Fig. 1. Classical auxin signaling mechanism. (A) At low auxin level, the Aux/IAA proteins form dimerization with ARFs, which results in inhibition of the transcriptional activity of the ARF genes. (B) At high auxin level, auxin promotes ubiquitination and de...
in yeast and quantitative IAA binding assays using purified proteins showed that different combinations of TIR1 and Aux/IAA proteins form co-receptor complexes with a wide range of auxin-binding affinities. Auxin affinity seems to be largely determined by the Aux/IAA. As there are 6 TIR1/AUXIN...
Generally, AUX/IAA TFs can interact with ARF TFs and form heterodimers, responding to auxin signals. Next, we constructed multiple yeast two-hybrid systems of ZmIAA29 with mounts of ZmARFs. However, only a few pairs of reactions were positive. ZmIAA29 with ZmARF2, ZmARF7, ZmARF15, ZmARF1...
the mutant showed a strong ability to form lateral branch meristems, with the marked characteristic that lateral branches always originated at cotyledon nodes. Another characteristic was that the first trifoliate leaf grew opposite to the second (Figure 1F). The mutant was severely defective in later...
extracts were dissolved in 50 μL of methoxyamine hydrochloride (MOX, Sigma Aldrich, St. Louis, MO, USA) in pyridine (20 mg/mL,w/v), rigorously mixed (vortexed), and sonicated for 1 min in an ultrasonic bath. MOX reacts with both aldehydes and ketones to form oxim methyl ethers and ...
which leads to the re-differentiation of the adventitious root, initiating cells to form procambium cells; then, the procambium cells undergo successive divisions to form adventitious root primordia; and finally, adventitious root primordia begin to appear and elongate to form adventitious roots [4,...