ArticleCASPubMedPubMed CentralGoogle Scholar Zhang, Z., McCaffery, J. M., Spencer, R. G. & Francomano, C. A. Hyaline cartilage engineered by chondrocytes in pellet culture: histological, immunohistochemical and ultrastructural analysis in comparison with cartilage explants.J. Anat.205, 229–237...
Under normal culture conditions, conditioned medium from ADSCs reduced the production of TNF-α, NO and prostaglandin E2, and the activation of nuclear factor-kappaB in blood-derived monocytes decreased their degranulation, phagocytic activity and migratory ability. Notably, using next-generation ...
Although in vitro culture models demonstrate hepatic tissue function, but these models could not completely restore the vital organ function such as metabolism and synthesis. All these systems mainly concentrate on the hepatocyte cells expansion. To achieve fully functional liver tissue, we need all ce...
7 Materials and Methods Cell culture and sample collection. Eight NPC cell lines 5-8F, 6-10B, CNE2, CNE1, C666-1, HONE1, HNE1 and SUNE1 were obtained from Cancer Research Institute of Southern Medical University, Guangzhou, China, and maintained in RPMI 1640 medium supplemented with 10...
However, the recellularization of human scaffolds has been poorly investigated, only Baker and Collaborators [103] reported the 3D-culture of human preadipocytes on acellular omentum. The study aimed at defining the role of the visceral adipose tissue ECM in regulating adipocyte metabolism in ...
Heart-on-a-chip using human iPSC-derived cardiomyocytes with an integrated vascular endothelial layer based on a culture patch as a potential platform for drug evaluation. Biofabrication 2022, 15, 015010. [CrossRef] Polymers 2023, 15, 1177 22 of 22 139. Criscione, J.; Rezaei, Z.; ...
(A) BLI analysis was performed at three days of culture and intensity of bioluminescence was determined. Data are expressed as the ratio of the BLI signal of the SOD2 group vs. mock control group. Data are expressed as mean ± SEM of three different independent experiments performed in ...
Preparation of Liquid Extracts of Each Dialyzed Silk-GMA Solution The biosafety test for the liquid extract of dialyzed Silk-GMA samples was prepared so that 1 g of Silk-GMA sponge of each dialysis period was mixed with 10 mL of 10% FBS containing a DMEM-high glucose culture medium, and ...
2.7. Cell Differentiation Assay Proliferating CTR and MSG SVF cells (having reached 70%–80% confluence after 5–6 days of culture) were induced to differentiate by the addition of a differentiation mix containing 5 µg/mL INS, 0.25 µM DXM, 0.5 mM 3-isobutyl-L-methylxanthine (IBMX) ...
Selection of reference genes for normalization of quantitative real-time PCR in organ culture of the rat and rabbit intervertebral disc. BMC Res. Notes 2011, 4, 162. 15. Pombo-Suarez, M.; Calaza, M.; Gomez-Reino, J.J.; Gonzalez, A. Reference genes for normalization of gene expression ...