Interaction between GC Box Binding Factors and Smad Proteins Modulates Cell Lineage-specific α2(I) Collagen Gene Transcription Inagaki Y,Nemoto T,Nakao A,et al.Interaction between GC box binding factors and Smad proteins modulates cell lineage-specific alpha 2(I)collagen gene ... Yutaka,Inagaki,...
Glycated albumin activates PAI-1 transcription through Smad DNA binding sites in mesangial cells. Am J Physiol, 2004, 287: F665-F672.Hyun Soon Lee, Kyung Chul Moon, Chi Young Song, et al. Glycated albumin activates PAI-1 transcription through Smad DNA binding sites in mesangial cells[J]....
Gel mobility shift assays showed protein phosphorylation-dependent binding activity in fibroblast nuclear extracts specific for this sequence; TGF-β treatment strongly stimulated the formation of this DNA-protein complex. Smad was identified as a component of the CAGACA-binding transcription complex in ...
The phosphorylation of Smad was repressed with the inhibitor, and the luciferase activity was cancelled using a mutant construct of Smad binding site of α2(I) collagen gene. However, the MAPK pathways, p38, ERK1/2 and JNK, were not affected with specific inhibitors or siRNA. The data ...
Lnc-Smad3 prevents Ash1l binding to the Smad3 promoter. Given the inverse expression of lnc-Smad3 and Smad3 during iTreg cell polarization, we next examined the epigenetic profile in lnc-Smad3 and Smad3 loci. With CHIP experiments, we found that with TGF-b stimulation, the H3K4me3 and ...
This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE). The protein acts as a tumor suppressor and inhibits epithelial cell proliferation. It may also have an inhibitory effect on tumors by reducing angiogenesis and increasng blood...
Binding of miR21 to the 3'untranslated region (UTR) of Smad7 was verified by dualluciferase assay. The authors observed that, in activated HSCs, expression of miR21 was significantly increased in a timedependent manner, while expression of Smad7 mRNA and protein was significantly reduced. In ...
Phosphorylated Smad2 www.chinaphar.com Liu Q et al npg 945 and Smad3 subsequently form a heteromeric complex with co-Smad and then translocate to the nucleus for DNA binding and transcriptional regulation[12]. To date, numerous studies have suggested the TGF-β/Smad pathway as promising anti-...
Cells were co-transfected with 0.5 μg of the SBE-LUC reporter construct (containing four DNA binding sites for Smad3/Smad4), and either 0.5 μg of the vector encoding wild-type Smad3, mutated Smad3 (Smad3D) or the empty expression vector pEXL, along with 0.5 μg of CMV-βgalactosidas...
Serine-204 in the linker region of Smad3 mediates the collagen-I response to TGF-β in a cell phenotype-specific manner.pdf 2015-05-30上传 Serine-204 in the linker region of Smad3 mediates the collagen-I response to TGF-β in a cell phenotype-specific manner ...