DefinitionDefinitionSBE; Was initially defined by a consensus sequence of two inverted repeats of GTCT. More recent data suggest that a single GNCN repeat may also be sufficient for Smad-DNA binding.Springer Berlin HeidelbergEncyclopedia of Cancer...
DNA-affinity precipitation analysis revealed that c-Ski enhances the binding of Smad2 and 4, and to a lesser extent Smad3, to both CAGA and TGF-1 inhibitory element probes. A c-Ski mutant, which is unable to interact with Smad4, failed to enhance the binding of Smad complex on these ...
DNA-affinity precipitation analysis revealed that c-Ski enhances the binding of Smad2 and 4, and to a lesser extent Smad3, to both CAGA and TGF-1 inhibitory element probes. A c-Ski mutant, which is unable to interact with Smad4, failed to enhance the binding of Smad complex on these ...
Figure 3. Bacterially expressed Smad proteins directly bind to the Smad-responsive element. A, binding of Smad proteins to the 120-bp MluNI-AflII region. Smads 1–5 were expressed as full-length or C-terminal truncated (ΔMH2) GST-fusion proteins in E. coli. The purified proteins were in...
Similar results were observed with region −387/−264 of the mGnRHR gene promoter, although the magnitude of the response to GnRH agonist was greater, because this region contains both the −387/−308 region of interest as well as the previously described bipartite GnRH-response element,...
**Po0.01. LTBP-4, latent TGF-β-binding protein 4; SBE, SMAD-binding element; TGF-β, transforming growth factor-β. in the upstream of TGF-β signaling pathway LTBP-4 has been proved to regulate the export of TGF-β into ECM.34 In our study, a decreased level of TGF-β was ...
Transforming Growth Factor betaDNA-Binding ProteinsHomeodomain ProteinsWe have investigated the regulation of the activin-inducible distal element (DE) of the ... S Germain,M Howell,GM Esslemont,... - 《Genes & Development》 被引量: 642发表: 2000年 Smad3 and Smad4 Mediate Transcriptional Activat...
Also provided are methods for using the Smad binding elements in methods of screening for compounds which bind the Smad binding element and in methods for modulating the binding of Smad proteins to Smad binding elements, e.g. to modulate TGF beta signal transduction. Further, the identification ...
Also provided are methods for using the Smad binding elements in methods of screening for compounds which bind the Smad binding element and in methods for modulating the binding of Smad proteins to Smad binding elements, e.g. to modulate TGFβ signal transduction. Further, the identification of ...
Also provided are methods for using the Smad binding elements in methods of screening for compounds which bind the Smad binding element and in methods for modulating the binding of Smad proteins to Smad binding elements, e.g. to modulate TGFβ signal transduction. Further, the identification of ...