At cryogenic temperature, a large number of hydration water molecules around proteins have been identified using X-ray crystal structure analyses, as described in Chap. 2 . However, at a glance, it seems that there are no rules governing their distribution. In this chapter, we attempt to ...
Also, the disruption of water structure around the rest of the protein helps prevent the adsorbed protein becoming covered by further ice growth. 展开 关键词: basic biological sciences antifreeze aqueous solutions hydration proteins solvation spruces water ...
Integrating our previous findings on α-helical proteins, we conclude the following: (1) The hydration dynamics is highly heterogeneous around the protein surface of both α-helical and β-sheet proteins. The outer-lay... 展开 DOI: 10.1021/jacs.6b12463 被引量: 2 ...
This corresponds to exclusion of the molar ectoine solvent by about one molecular layer (about 3 Å thick) of dense water around the protein surface. Neutron membrane diffraction shows ectoine is excluded from the surface of purple membranes (PM). The D16 diffractometer at the ILL was ...
k∗10−4сек−12 cm deep from the surface layer (refr.) k∗10−4 sec15 cm deep from the surface layer (refr.) k∗10−4 sec−1Surface layer (test) k∗10−4 sec−12 cm deep from the surface layer (test) k∗10−4 sec−15 cm deep from the surface ...
Prediction of hydration structures around hydrophilic surfaces of proteins by using the empirical hydration distribution functions from a database analysis. J. Phys. Chem. B 114, 4652–4663 (2010). 39. Nakasako, M. et al. Conformational dynamics of complementarity-determining region H3 of an ...
It is hypothesized that the nonfouling ability of both polyhydrophilic and polyzwitterionic materials are tightly correlated with a hydration layer near the surface [16], [17], [18], because a tightly bound water layer forms a physical and energetic barrier to prevent protein adsorption on the ...
Knowledge of water-mediated interactions obtained over a broad thermodynamic space of temperature, pressure, and additive concentration can be integrated to understand a variety of biophysical phenomena. I will illustrate this point using an application to pressure denaturation of proteins. 展开 ...
This is the mechanism by which the high surface charge on the oxide is reconciled with the quite low diffuse double layer potentials (zeta potentials). For other types of surfaces such as proteins, where there are few or no adsorbed ions at all, the modified Gouy–Chapman model [26], ...
Introduction The resident microbiome is an essential component of the human skin with considerable inter-individual variations1,2,3. The microbiome composition varies by layer of the skin and changes with skin barrier disruption4,5. The skin microflora maintains a homoeostasis by cross-talk with the...