In this chapter, an overview of hybridoma technology and the laboratory procedures used routinely for hybridoma generation are discussed and detailed in the following sections: cell fusion for hybridoma generation, antibody screening and characterization, hybridoma subcloning and mAb isotyping, as well as...
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monoclonal antibodies. They can be selected for predetermined applications by appropriate screening strategies and monoclonal antibodies are now finding wide use as reagents in specific diagnostic tasks. In another application, a monoclonal antibody linked to an appropriate column matrix, is used to bind...
by fusion of human lymphocytes with a lymphoma cell line (pref. mouse myeloma), screening and cloning the hybridoma for ani-Candida monoclonal antibody prodn. An Epstein-Bar... T Kawamura,YTSC Masuho,S Sawada,... - EP 被引量: 0发表: 1995年 HUMAN MONOCLONAL ANTIBODY AGAINST CANDIDA. ...
Jones V. E.: 1969. Rat 7S immunoglobulins: characterization of gamma 2- and gamma 1- anti-hapten antibodies.Immunology16: 589–599. PubMedCASGoogle Scholar Der Balian G., Slack J., Clevinger B., Bazin H., and Davie J. M: 1980. Subclass restriction of murine antibodies. III. Antigens...
In addition, the few human myeloma cell lines that do grow well in culture produce their own Ig molecules, causing background difficulties during screening. As an alternative to human hybridomas, researchers have created “humanized” mouse antibodies, chimeric Ig molecules in which the mouse V ...
Isolation and characterization of monoclonal antibodies specific for m74 gene product of mouse cytomegalovirus We selected hybridomas secreting Abs specific for MCMV antigens by screening using ELISA and flow cytometry. By gene transfection we created a transient ... F Skenderi 被引量: 1发表: 2011...
be reprogrammed with only a single transfection and screening step. Characterization of immunogenomic editing in hybridomas Generation of a PnP-mRuby hybridoma cell line Figure 1: Generation of plug-and-(dis)play-mRuby cells. We aimed to make the mRuby+cell line deficient for light chain ...
This article aims to establish an efficient assay for screening monoclonal antibodies (McAbs) against the membrane proteins of chicken embryo fibroblast (C... YY Yang,LQ Zhu,JF Yang,... - 《Journal of Integrative Agriculture》 被引量: 27发表: 2010年 Specificities and binding properties of 2 ...
YamadaK, IkedaI, SugaharaT, ShirahataS and MurakamiH (1989b) Screening of immunoglobulin production stimulating factor (IPSF) in foodstuffs using human-human hybridoma HB4C5 cells. Agric. Biol. Chem. 53: 2987–2991. Google Scholar YamadaK, IkedaI, SugaharaT, HashizumeS, ShirahataS and Murak...