The technique of in situ hybridization (ISH) is an elegant synthesis of molecular biology and cytology. In situ hybridization makes possible the detection and localization of specific nucleic acid sequences within tissue sections or whole mount preparations of single cells, organelles or chromosomes. ...
detecting and quantifying interspecies gene flow is surprisingly difficult. Until recent advances in sequencing technologies, scientists relied on phenotypic characters to study hybridization, which sometimes yielded results incongruent with molecular data. Furthermore, identification of hybrids is also difficult...
Thus, the interpreter not only should have expertise in molecular biology but also must be skilled in the morphologic assessment of histopathology and cytology specimens; in other words, this technique should be under the supervision of a pathologist who can include these morphologic findings in the...
In situ hybridization has become established as an essential method in the study of cell and molecular biology, and is a powerful technique to visualize host–pathogen interactions. In many laboratories, in situ hybridization is a routine procedure, particularly in the diagnosis of viral disease. A...
Background The genomes of higher plants are, on the majority, polyploid, and hybridisation is more frequent in plants than in animals. Both polyploidisatio... R Schmickl,MH J?Rgensen,AK Brysting,... - 《Bmc Evolutionary Biology》 被引量: 141发表: 2010年 Cryptic mitochondrial lineages in...
Quantitation of mRNA immobilized on nitrocellulose filters is an essential aspect of some studies in molecular biology. Hybridization of oligo(dT) 18 to the poly(A) tails of mRNA can be used to measure filter-bound mRNA and thus provides a basis for comparing abundance of specific mRNAs. Hybrid...
One of the most basic experiments in molecular biology is quantitative measurement of binding kinetics and thermodynamics between two bioactive molecules such as DNA, RNA, proteins and ligands. In particular, binding interactions between small-size oligonucleotides such as DNA and RNA are of both funda...
- 1995 - 333-334 - www.ingentaconnect.comdoi:10.1016/S0168-9525(00)89097-6
Advancements in molecular genetics have revealed that hybridization may be common among plants, animals, and fungi, playing a role in evolutionary dynamics and speciation. While hybridization has been well-documented in pathogenic fungi, the effects of these processes on speciation in fungal lineages wi...
SUSAN J.KARCHER, inMolecular Biology, 1995 NOTE A hybridization oven is an alternative to working with radioactivity in sealed plastic bags. With a hybridization oven the blot and solutions are placed in bottles, which are rotated in the oven. It is generally much easier to remove the radioac...