which is strongly expressed on the parent site of the boundary during the final stages of budding27. Furthermore, we assumed that Notch-signalling was blocked in the body-column of the parent polyp
For this, we blocked mitochondrial O2 consumption by addition of antimycin A and rotenone and activated NOX2 by addition of PMA. Similar to the WST-1 assay, Hv1-deficient cells only consumed approximately 30 % of O2 compared to WT, suggesting that production of intracellular NOX2-dependent ...
(a) Schematics of the F150A-WT linked dimer used to generate blocked hemichannels ({F150A}b-WT/BAO*). White diamond shows position of mutation. The affinity of the F150A subunit is expected to be higher than the WT subunit in both the BAO* and BA*B* states. (b) Proton current ...
ICLs represent a severe barrier to DNA replication, causing DSBs at blocked replication forks. To assess the impact of POLH and ATR on DNA replication and DSB formation, replication was analyzed by the BrdU incorporation assay, and DSB formation was tested by detec- WLRQ RI DŽ+$; ...