比如:ECGF:内皮细胞生长因子,可以显著提高HUVEC细胞周期中的S期细胞数量,促进细胞DNA合成,从而加速细胞的增殖速度;ECGF还能保持HUVEC细胞的正常生理功能,如血管生成能力。L-glutamine:在细胞培养中,尤其当细胞快速分裂时,L-谷氨酰胺可作为一种辅助能源。Heparin:肝素可以与某些生长因子结合,增加其稳定性,并促进...
中文名称:HUVEC-C[HUVEC] Cell:人脐静脉血管内皮细胞系英文名称:HUVEC-C[HUVEC] Cell 保存条件:液氮保存纯度规格:1x10(6)viable cells/ml 产品类别:实验化学品 "HUVEC-C[HUVEC] Cell:人脐静脉血管内皮细胞系 细胞形态:上皮细胞样 细胞生长:贴壁 细胞背景资料:脐静脉;内皮细胞 ...
Applied Sciences, 2020. 10(3). 2. Marin V, K.G., Grès S, Farnarier C, Bongrand P, Endothelial cell culture: protocol to obtain and cultivate human umbilical endothelial cells. J Immunol Methods, 2001 Aug 1. 254: p. 183-90.
HUVEC(HUV-EC-C) Cell<人脐静脉血管内皮细胞系> HRA-19细胞系;细胞传代方法:1:2-1:3传代;每周换液2-3次;细胞形态特性:详见细胞说明书;细胞生长特性:贴壁或悬浮,详见细胞说明书部分;细胞背景资料:详见相关文献介绍 U27KM细胞系;细胞传代方法:1:2-1:3传代;每周换液2-3次;细胞形态特性:详见细胞说明书;细...
Lonza offers HUVECs which have received additional product testing and are guaranteed to test positive for angiogenesis and vascular cell health markers,eNOS,Tie-2,VEGFr2, and Axl in the first passage out of cryopreservation. These cells have undergone the same testing as the non-screened cells an...
(1)内皮细胞基础培养基推荐:ScienCell(Cat.No.1001)基本成分介绍:永生化的人脐静脉内皮培养体系是专门为血管内皮细胞体外培养设计的最适于其生长的完全培养基。包含必需和非必需氨基酸、维生素、有机和无机化合物、激素、生长因子、微量矿物质和低浓度胎牛血清(5%)。该培养基的配方能够选择性的促进正常血管内皮...
1.制备纤维连接蛋白包被的培养皿(2 μg/cm2,推荐使用T-75)。首先添加10ml DPBS,(ScienCell,Cat#0303,无钙离子和镁离子)加入T75培养皿中,再加入150 μl纤维连接蛋白(Cat# 8248)。将培养皿放在37℃的培养箱中过夜(或至少2小时)。 ...
MACS® Cell Storage Solution 130-130-263 HUVEC细胞培养步骤[2] 细胞接种: 1.明胶包被:HUVEC细胞需在涂有2μg/cm2重组人纤连蛋白或1%明胶的聚苯乙烯瓶或孔中培养。使用2μg/cm2重组人纤连蛋白或1%明胶包被培养瓶或孔板,置于37℃孵育1h,在加入HUVEC细胞前使用无菌蒸馏水去重组人纤连蛋白或除明胶; ...
human umbilical vein endothelial cells (HUVEC) as a model to study cardiovascular disease: A review." Applied Sciences 10.3 (2020): 938.[4] Liao, Danying, et al. "CRISPR-mediated deletion of the PECAM-1 cytoplasmic domain increases receptor lateral mobility and strengthens endothelial cell ...
[4] Liao, Danying, et al. "CRISPR-mediated deletion of the PECAM-1 cytoplasmic domain increases receptor lateral mobility and strengthens endothelial cell junctional integrity." Life sciences 193 (2018): 186-193.[5] Schwefel, Konrad, et al. "Fibronectin rescues aberrant phenotype of endothelial...