huPBMC-NCG小鼠是将人外周血单个核细胞,移植到重度免疫缺陷小鼠NCG体内,从而获得的重建人免疫系统的NCG小鼠模型。在NCG小鼠上进行的huPBMC免疫重建效率高,速度快,重建后以淋巴T细胞为主,可用于多种肿瘤免疫药物的评价。
基于huPBMC-NCG小鼠体内GvHD药效试验 huPBMC-NCG小鼠可用于GvHD治疗。利用Donor筛选构建较短生存期的小鼠模型,通过使用阿巴西普治疗huPBMC-NCG小鼠,结果显示该药物有效延长了huPBMC-NCG小鼠的生存周期,且随着给药剂量的增加,生存周期明显延长,...
huPBMC-NCG小鼠是将人外周血单个核细胞(Peripheral Blood Mononuclear Cell, PBMC)移植到重度免疫缺陷小鼠NCG体内,从而获得的重建人免疫系统的NCG小鼠模型。 huPBMC-NCG小鼠人源化模型实验设计流程图 药康优势 集萃药康自主研发的NCG小鼠是使用基因编辑技术敲除了NOD/ShiltJNju小鼠的Prkdc及Il2rg基因而获得的重度免疫缺...
图1. 人源PBMC重建后小鼠体重变化统计 NCG和NCG-MHC-dKO小鼠在注射人源 PBMC后,观察小鼠体重变化,结果发现敲除MHCI/MHCII后可显著改善PBMC重建后因GvHD发生导致的小鼠体重下降情况。 图2. 人源PBMC重建后小鼠生存情况统计 NCG和NCG-MHC-dKO小鼠在注射人源 PBMC后,观察小鼠生存变化,结果发现敲除MHCI/MHCII后可显著...
【huPBMC-NCG项目介绍】 huPBMC-NCG小鼠是将人外周血单个核细胞(Peripheral Blood Mononuclear Cell, PBMC)移植到重度免疫缺陷小鼠NCG体内,从而获得的重建人免疫系统的NCG小鼠模型。 分别将来源于供体F和供体G的huPBMC注射到NCG小鼠中,分析huPBMC-NCG免疫重建小鼠的存活时间。由于植入的PBMC为异源免疫细胞,huPBMC-NCG...
Meanwhile, irradiated huPBMC-NCG mice presented less variability in the kinetics of GVHD and the frequency of this disease in comparison with unconditioned counterparts, thus providing a more stable model for human GVHD studies. For preclinical studies, we found immunomodulatory drugs including ...
Conclusions: The huPBMC-NCG mouse model allows to study interaction between human immune component and ICI therapy in a preclinical setting. According to those preliminary data, 64Cu-DOTHA2-FAPI-04-PET appears to be better suited than FDG-PET to adequately follow-up ICI therapy.Michel Paquette...