Hormones in human blood, detection and assayGuidelines for submitting commentsPolicy: Comments that contribute to the discussion of the article will be posted within approximately three business days. We do not accept anonymous comments. Please include your email address; the address will not be ...
Generation of human blood vessel organoids Fig. 1: Generation of human blood vessel organoids (BVOs). Full size image IPS-derived ECs are highly glycolytic Fig. 2: Human iPS cell differentiation toward ECs. Full size image Fig. 3: Comparison of glycolytic rates in HUVEC, iPS-ECs and iPS...
Genetic factors modifying the blood metabolome have been investigated through genome-wide association studies (GWAS) of common genetic variants and through exome sequencing. We conducted a whole-genome sequencing study of common, low-frequency and rare variants to associate genetic variations with blood...
In contrast to peripheral blood mononuclear cell (PBMC) culture, a whole blood assay (WBA) provides a more physiological environment, which may provide a broader assessment of serum biomarker, biosignature profiles. Twenty-three asymptomatic individuals with M. tuberculosis infection were recruited. ...
October 28, 2024 [Journal of Clinical Investigation] Scientists observed an upregulation of patients' inflammatory gene expression signature in whole blood, mimicking monogenic inborn errors underlying autoinflammation. Remarkably, these patients had constitutively activated monocytes that secreted 10 to 100 ...
albicans), agar with 5% human blood (group B streptococci), and chocolate II agar (N. gonorrhoeae) were included. A CDC anaerobic 5% sheep blood agar plate was streaked to isolate obligate and facultative anaerobes, as well as microaerophilic bacteria, using a Gas Pak EZ anaerobic system. ...
2.5. Cytotoxicity assay 2.5.1. Isolation of Peripheral Blood Mononuclear Cell (PBMC) The ficoll density gradient separation of whole blood is the most commonly used procedure for separation of mononuclear cells [28]. Briefly, 0.5 mL of heparin was added to 5 mL of blood in a falcon tube and...
The blood-nerve barrier (BNB), formed by tight junction-forming microvessels within peripheral nerve endoneurium, exists to regulate its internal microenvironment essential for effective axonal signal transduction. Relatively little is known about the unique human BNB molecular composition. Such knowledge...
Little is known about mechanics underlying the interaction among platelets during activation and aggregation. Although the strength of a blood thrombus has likely major biological importance, no previous study has measured directly the adhesion forces of single platelet-platelet interaction at different acti...
In the present study, our aim was to investigate the expression of mRNA and protein for somatostatin receptor types-1, -2, -3, -4, -5 (SSTR-1–5) in human UM tissue samples and in OCM-1 and OCM-3 human UM cell lines by qRT-PCR, western blot and ligand competition assay. The ...