Whole cell assayFan FrankJi YinduoKallender HowardLi TongMcdevitt DamienWO2001007061A1 1999年7月27日 2001年2月1日 Smithkline Beecham Corp Whole cell assay
WHOLE CELL ASSAY 专利名称：WHOLE CELL ASSAY 发明人：FAN, Frank,JI, Yinduo,KALLENDER, Howard,LI, Tong,MCDEVITT, Damien 申请号：US1999017001 申请日：19990727 公开号：WO01/007061P1 公开日：20010201 专利内容由知识产权出版社提供 摘要：This invention relates to newly developed methods for discovering...
WHOLE CELL ASSAY FOR CATHEPSIN KWHOLE CELL ASSAY FOR CATHEPSIN Knot available for EP1214444of corresponding document: WO0068414 The present invention relates to a whole cell assay for cathepsin K. The present invention is useful for determining cathepsin K activity in mammalian cell systems and for...
Microbial whole-cell biosensors (MWCBs) are analytical, biological devices with a genetic element that acts as a sensor, capable of recognizing an analyte or a toxin, together with a reporter protein, capable of generating a quantifiable output signal (Daunert et al., 2000; ...
In the assay, plasma membrane integrity remained intact as assessed by release of lactate dehydrogenase from the intracellular environment and the exclusion of cell impermeable peptide inhibitors, despite the induction of an apoptotic state. The method described here is a robust tool to support drug ...
The nine different whole-cell assay systems (three reporter strains × three QS proteins) were evaluated for their applicability and reliability by studying quantitative responses to various furanones, which are potent inhibitors of the LuxR family proteins. These results demonstrate that the cell-...
Immune response indicators include but are not limited to: antibody titer or specificity, as detected by an assay such as enzyme-linked immunoassay (ELISA), bactericidal assay, flow cytometry, immunoprecipitation, Ouchter-Lowny immunodiffusion; binding detection assays of, for example, spot, Western...
5.2 Isotope efflux assay The radioisotope efflux assay is a rapid and easy method to quantitate alterations in ionic conductances, as well as to determine the release of small organic osmolytes from cultures of cells (Tomassen et al, 2004; Vaandrager et al., 1991; Fig. 19.2). As a tracer...
DOTAP and were irradiated to prevent their replication and to enhance antigen presentation. We found that this pFAP transfectant expressed bioactive FAPin vitro. In this assay, we used murine melanoma B16F10 cells, Lewis lung carcinoma LL/2 cells and colon carcinoma CT26 cells to generate tumor...
the cell suspensions. All MHETase activity for the intracellular construct was in the supernatant, consistent with release due to cell lysis. We suggest that some caution is warranted in interpreting display activity results unless the whole-cell catalyst is washed prior to assay. Activity in the ...