LB811 Exosomes from human neonatal fibroblasts conditioned media play an important role in skin rejuvenationdoi:10.1016/j.jid.2021.07.126K. KadoyaS. WheelerR. KalaR. MehtaElsevier BVJournal of Investigative Dermatology
The abbreviations used are: hESC, human embryonic stem cell; DDA, data-dependent acquisition; ACN, acetonitrile; bFGF, basic fibroblast growth factor; CM, conditioned medium; MuD-PIT, multi-dimensional protein identification technology; FA, formic acid; IE, iterative exclusion; IGF, insulin-like ...
A proteome analysis of conditioned media from human neonatal fibroblasts used in the maintenance of human embryonic stem cells. Proteomics 2005; 5:978-989. 54 Lim JW, Bodnar A. Proteome analysis of conditioned medium from mouse embryonic fibroblast feeder layers which support the growth of human ...
Human BJ fibroblast cells (ATCC®CRL-2522) were plated so as to be 30–60% confluent for transduction two days later (1 × 105cells per well of a six-well plate). Cells were transduced using the Cytotune™ 2.0 Sendai reprogramming kit (Invitrogen) per manufacturer’s instructions, ...
Moreover, the SASP of old cardiomyocytes reduced the proliferation of neonatal fibroblasts and increased the expression of α-smooth muscle actin (αSMA), an indicator of myofibroblast activation[11]. In vivo work corroborates the detrimental effect of the SASP on cardiac repair and regeneration. ...
Fibroblast growth factor 2 HRP: Horseradish peroxidase iPSCs: Induced pluripotent stem cells IPA: Ingenuity Pathway Analysis ICAM: Intercellular adhesion molecule IL: Interleukin iECs: IPSC-endothelial cells iPLCs: IPSC-pericyte-like cells MCP-1/CCL2: Monocyte chemoattractant protein 1 NDM...
ROCKi Y-27632, added to the difficult-to-proliferate keratinocytes, traditionally cultured in the presence of lethally irradiated mouse fibroblast feeder 3T3-J2 cells, rapidly changed keratinocyte morphology and induced robust proliferation, providing a nearly infinite source of undifferentiated keratinocytes ...
Although in this study, iPSC-derived mouse microglia were not studied, we compared the LPS responses of primary neonatal microglia with the human iMGL cells, which may also exhibit young phenotypes. Our results should be interpreted in the light of the wide plethora of the experimental setups ...
(Primorigen Biosciences; Madison, WI), fed daily with mTeSR1 as previously described41and supplemented with 20% mouse embryonic fibroblast (MEF)-conditioned media and 20 ng/ml fibroblast growth factor (FGF), and maintained in hypoxic culture conditions at 37 °C, 4% O2/5% CO2. iPSC were ...
“aFGF” means acidic fibroblast growth factor (sometimes also referred to as FGF1). As used herein, the term “BMP” means Bone Morphogenic Protein, preferably of human and/or recombinant origin, and subtypes belonging thereto are e.g. BMP4 and BMP2. As used herein, the term “HGF” ...